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Updated: Jan 20, 2026
Preparation of Myxococcus xanthus Samples for Live-Cell Imaging
Published on: November 28, 2025
Myxochelin- and Pseudochelin-Derived Lipoxygenase Inhibitors from a Genetically Engineered Myxococcus xanthus Strain
Angela Sester1, Lea Winand, Simona Pace2
1Leibniz Institute for Natural Product Research and Infection Biology e.V. , Hans-Knöll-Institute , Beutenbergstraße 11a , 07745 Jena , Germany.
Abstract:
Precursor-directed biosynthesis was used to introduce selected aryl carboxylic acids into the pseudochelin pathway, which had recently been assembled in Myxococcus xanthus. Overall, 14 previously undescribed analogues of the natural products myxochelin B and pseudochelin A were generated and structurally characterized. A subset of 10 derivatives together with their parental molecules were evaluated for their activity toward human 5-lipoxygenase. This testing revealed pseudochelin A as the most potent 5-lipoxygenase inhibitor among the naturally occurring compounds, whereas myxochelin A is the least active. Replacement of the catechol moieties in myxochelin B and pseudochelin A affected the bioactivity to different degrees.
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