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Related Experiment Video

Updated: Jan 20, 2026

In Vivo Imaging of the Mouse Retina Using Optical Coherence Tomography
04:08

In Vivo Imaging of the Mouse Retina Using Optical Coherence Tomography

Published on: May 29, 2025

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Calibration-free time-stretch optical coherence tomography with large imaging depth.

Lei Zhang, Liao Chen, Zihui Lei

    Optics Letters
    |August 30, 2019
    PubMed
    Summary

    A new calibration-free time-stretch optical coherence tomography (TS-OCT) uses optical dispersion compensation, significantly reducing processing time and enhancing imaging depth for biological tissues.

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    Area of Science:

    • Biomedical Optics
    • Optical Engineering
    • Medical Imaging Technology

    Background:

    • Time-stretch optical coherence tomography (TS-OCT) typically requires digital calibration, which is time-consuming and can limit imaging speed.
    • Higher-order dispersion in optical systems can degrade image quality and reduce imaging depth.

    Purpose of the Study:

    • To develop and demonstrate a calibration-free TS-OCT system using an optical higher-order dispersion compensation scheme.
    • To improve data processing efficiency, sensitivity, and imaging depth compared to traditional TS-OCT methods.

    Main Methods:

    • Implemented an optical higher-order dispersion compensation scheme to replace digital calibration in TS-OCT.
    • Acquired raw data that could be directly Fourier transformed, eliminating the need for digital calibration steps.

    Main Results:

    • Achieved an 82% reduction in data processing time compared to digital calibration methods.
    • Increased sensitivity roll-off by 2.6 times (to 6.91 mm/dB) and effective imaging depth by 14.2% (to 16 mm).
    • Demonstrated in vivo biological tissue imaging with a single-shot A-scan rate approaching 19 MHz.

    Conclusions:

    • The optical higher-order dispersion compensation scheme offers a calibration-free approach for TS-OCT.
    • This method significantly enhances processing speed, imaging depth, and sensitivity, enabling real-time 3D imaging with improved quality.