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Related Concept Videos

Microfluidics in Assessing Platelet Function06:47

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Multiple comparison test, abbreviated as MCT, is a post hoc analysis generally performed after comparing multiple samples with one or more tests. An MCT will help identify a significantly different sample among multiple samples or a factor among multiple factors.
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Related Experiment Video

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Microfluidics in Assessing Platelet Function
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Platelet function testing in pigs using the Multiplate® Analyzer.

Sarah Heringer1, Lisa Kabelitz1, Martin Kramer2

  • 1Department of Diagnostic and Interventional Neuroradiology, RWTH Aachen University Hospital, Aachen, Germany.

Plos One
|August 30, 2019
PubMed
Summary
This summary is machine-generated.

The Multiplate® Analyzer effectively assesses platelet function in pigs, confirming that acetylsalicylic acid (ASA) and clopidogrel reliably inhibit platelet activity. This method can detect non-responders, crucial for endovascular research.

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Area of Science:

  • Veterinary Medicine
  • Pharmacology
  • Cardiovascular Research

Background:

  • Pigs are a common animal model in endovascular research.
  • Limited data exists on analyzing platelet inhibition in pigs using the Multiplate® Analyzer.

Purpose of the Study:

  • Evaluate the Multiplate® Analyzer for porcine platelet function analysis.
  • Assess the reliability of inducing platelet inhibition with acetylsalicylic acid (ASA) and clopidogrel in pigs.
  • Determine if non-responders to antiplatelet drugs can be identified.
  • Compare ASA effectiveness via oral vs. intravenous routes.
  • Investigate differences between domestic (German Landrace) and miniature pigs.

Main Methods:

  • Analyzed platelet function in 36 unmedicated and 32 medicated pigs (domestic and miniature breeds).
  • Administered ASA (oral/intravenous) and clopidogrel (oral) to induce platelet inhibition.
  • Utilized the Multiplate® Analyzer (ASPI and ADP tests).

Main Results:

  • Multiplate® Analyzer results in pigs were comparable to human data.
  • ASA significantly decreased ASPI test values irrespective of administration route.
  • Combined ASA and clopidogrel significantly decreased ADP test values.
  • Both ASA and clopidogrel effectively inhibited platelet function in pigs.
  • One pig showed no response to clopidogrel.
  • No significant differences were found between domestic and miniature pigs.

Conclusions:

  • The Multiplate® Analyzer is suitable for assessing porcine platelet function.
  • ASA and clopidogrel reliably inhibit platelet function in pigs, with potential to detect non-responders.
  • Both oral and intravenous ASA, and oral clopidogrel, are effective in pigs.
  • No breed-specific differences were observed in this study.