Developing an Impedance Based Equation for Fat-Free Mass of Black Preadolescent South African Children

Bioelectrical impedance analysis (BIA) is a practical alternative to dual-energy X-ray absorptiometry (DXA) for determining body composition in children. Currently, there are no population specific equations available for predicting fat-free mass (FFM) in South African populations. We determined agreement between fat-free mass measured by DXA (FFM_DXA) and FFM calculated from published multi-frequency bioelectrical impedance prediction equations (FFM_BIA); and developed a new equation for predicting FFM for preadolescent black South African children. Cross-sectional data on a convenience sample of 84 children (mean age 8.5 ± 1.4 years; 44 {52%} girls) included body composition assessed using Dual X-ray Absorptiometry (FFM_DXA) and impedance values obtained from the Seca mBCA 514 Medical Body Composition analyzer used to calculate FFM using 17 published prediction equations (FFM_BIA). Only two equations yielded FFM estimates that were similar to the DXA readings (p > 0.05). According to the Bland-Altman analysis, the mean differences in FFM (kg) were 0.15 (LOA: -2.68; 2.37) and 0.01 (LOA: -2.68; 2.66). Our new prediction equation, F F M = 105.20 + 0.807 × S e x + 0.174 × W e i g h t + 0.01 × R e a c t a n c e + 15.71 × log ( R I ) , yielded an adjusted R² = 0.9544. No statistical shrinkage was observed during cross-validation. A new equation enables the BIA-based prediction of FFM in the assessment of preadolescent black South African children.