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Tracking the Mammary Architectural Features and Detecting Breast Cancer with Magnetic Resonance Diffusion Tensor Imaging15:48

Tracking the Mammary Architectural Features and Detecting Breast Cancer with Magnetic Resonance Diffusion Tensor Imaging

We describe how to obtain parametric and vector maps of the diffusion tensor of the breast using magnetic resonance imaging. The protocol and final output following imaging processing are tailored for tracking breast architectural features and detecting breast malignancy.
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Guidelines and Experience Using Imaging Biomarker Explorer (IBEX) for Radiomics10:17

Guidelines and Experience Using Imaging Biomarker Explorer (IBEX) for Radiomics

We describe IBEX, an open-source tool designed for medical imaging radiomics studies, and how to use this tool. In addition, some published works that have used IBEX for uncertainty analysis and model building are...
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Diffusion Imaging in the Rat Cervical Spinal Cord10:46

Diffusion Imaging in the Rat Cervical Spinal Cord

The goal of this protocol is to obtain high-quality diffusion weighted magnetic resonance imaging (DWI) of the rat spinal cord for noninvasive characterization of tissue microstructure. This protocol describes optimizations of the MRI sequence, radiofrequency coil, and analysis methods to enable DWI images free from...
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Measuring Diffusion Coefficients via Two-photon Fluorescence Recovery After Photobleaching07:00

Measuring Diffusion Coefficients via Two-photon Fluorescence Recovery After Photobleaching

In this article we will describe the procedure for measuring diffusion coefficients using multi-photon fluorescence recovery after photobleaching. We will begin by aligning the laser along the optical path to the sample and determining the proper experimental parameters, then continue generating and finally fitting fluorescence recovery...
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Easy Measurement of Diffusion Coefficients of EGFP-tagged Plasma Membrane Proteins Using k-Space Image Correlation Spectroscopy11:43

Easy Measurement of Diffusion Coefficients of EGFP-tagged Plasma Membrane Proteins Using k-Space Image Correlation Spectroscopy

This paper provides a step by step guide to the fluctuation analysis technique k-Space Image Correlation Spectroscopy (kICS) for measuring diffusion coefficients of fluorescently labeled plasma membrane proteins in live mammalian...
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Assessing Spinal Cord Compression Using Diffusion Tensor Imaging05:20

Assessing Spinal Cord Compression Using Diffusion Tensor Imaging

Source: Zheng, W. et. al., Diffusion Tensor Magnetic Resonance Imaging in Chronic Spinal Cord Compression. J. Vis. Exp. (2019)This video demonstrates diffusion tensor imaging in assessing chronic spinal cord compression. By analyzing water diffusion patterns, fractional anisotropy, and apparent diffusion coefficient values, the findings reveal axonal damage in compressed regions. The findings provide critical insights into spinal cord pathology and structural...
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