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Deploying Community Scientists to Conduct Nondestructive Genetic Sampling of Rare Butterfly Populations07:17

Deploying Community Scientists to Conduct Nondestructive Genetic Sampling of Rare Butterfly Populations

Here, we present a straightforward protocol for noninvasive genetic sampling of butterfly populations based on the field collection of residual egg debris. It can be used to confirm species identity and quantify genetic variation. This protocol can be easily adapted to broader groups for community science...
1.9K
Establishment and Genetic Manipulation of Murine Hepatocyte Organoids14:54

Establishment and Genetic Manipulation of Murine Hepatocyte Organoids

A long-term ex vitro 3D organoid culture system was established from mouse hepatocytes. These organoids can be passaged and genetically manipulated by lentivirus infection of shRNA/ectopic construction, siRNA transfection and CRISPR-Cas9...
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Dermoscopy Aids in the Diagnosis of Discoid Lupus Erythematosus05:39

Dermoscopy Aids in the Diagnosis of Discoid Lupus Erythematosus

Herein, we present a protocol to demonstrate the utility of dermoscopy in rheumatic diseases. In addition, we described the dermoscopic manifestations of discoid lupus erythematosus skin...
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Visualizing Motion Patterns in Acupuncture Manipulation08:18

Visualizing Motion Patterns in Acupuncture Manipulation

Here, we present a protocol for using the Acupuncture Manipulation Education System (AMES) in the training of acupuncture manipulation skills using phantom...
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In situ Protocol for Butterfly Pupal Wings Using Riboprobes06:19

In situ Protocol for Butterfly Pupal Wings Using Riboprobes

In order to examine gene expression in the pupal wing tissue of Bicyclus anynana, we present an optimized protocol for in situ hybridizations using riboprobes. We also provide guidelines for the further optimization of this protocol for use in pupal wings of other Lepidopteran...
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Genetic Manipulation of the Plant Pathogen Ustilago maydis to Study Fungal Biology and Plant Microbe Interactions11:42

Genetic Manipulation of the Plant Pathogen Ustilago maydis to Study Fungal Biology and Plant Microbe Interactions

We describe a robust gene replacement strategy to genetically manipulate the smut fungus Ustilago maydis. This protocol explains how to generate deletion mutants to investigate infection phenotypes. It can be extended to modify genes in any desired way, e.g., by adding a sequence encoding a fluorescent protein...
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