The Validation of Neogen Reveal® Q+ for DON

Area of Science:

• Agricultural Science
• Food Safety
• Analytical Chemistry

Background:

• The Reveal® Q+ for DON is an immunochromatographic assay designed for the quantitative determination of deoxynivalenol (DON) in various grain matrices.
• Deoxynivalenol (DON) is a significant mycotoxin that can contaminate staple crops, posing risks to animal and human health.

Purpose of the Study:

• To validate the performance of the Reveal Q+ for DON assay for the accurate quantification of deoxynivalenol.
• To assess the assay's efficacy across a wide range of commodities, including naturally contaminated and spiked samples.

Main Methods:

• Validation of the immunochromatographic test for deoxynivalenol (DON) determination.
• Inclusion of matrix testing, limit of detection (LOD), linearity, selectivity, robustness, and stability studies.
• Comparison of test results with reference values and independent laboratory testing for corroboration.

Main Results:

• The assay demonstrated reliable quantification of DON with high recovery rates (90-104%) and excellent linearity (R² = 0.999).
• Selectivity testing confirmed no cross-reactivity with other mycotoxins, and stability studies established an 18-month shelf life.
• The limit of quantification (LOQ) was determined to be 0.042 ppm in corn and 0.11 ppm in wheat.

Conclusions:

• The Reveal Q+ for DON test is a validated Performance Tested Method for deoxynivalenol determination in corn, wheat, and other grains.
• The assay offers advantages such as simple aqueous extraction, procedural ease, minimal equipment needs, and rapid results, making it suitable for routine analysis.