Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Preparing a Mice Model of Severe Acute Pancreatitis via a Combination of Caerulein and Lipopolysaccharide Intraperitoneal Injection07:38

Preparing a Mice Model of Severe Acute Pancreatitis via a Combination of Caerulein and Lipopolysaccharide Intraperitoneal Injection

1.2K
Intraperitoneal drug administration is a safe and effective non-invasive approach for inducing pancreatic injury. This study compared five distinct intraperitoneal injection protocols on mice to induce varying degrees of pancreatic injury and established a model of severe pancreatic injury to investigate the pathological changes and treatment strategies for severe acute pancreatitis...
1.2K
Immunodeficiency Diseases01:25

Immunodeficiency Diseases

2.0K
Immunodeficiency disorders are conditions in which the immune system's ability to fight infectious disease and cancer is compromised or entirely absent. The immune system comprises a complex network of cells, tissues, and organs that work together to protect the body from potentially harmful invaders. When this system is deficient or not functioning properly, it leaves the body susceptible to infections, diseases, or other complications.
There are three main causes of immunodeficiency...
2.0K
How to Obtain Reliable Visual Event-related Potentials in Newborns07:39

How to Obtain Reliable Visual Event-related Potentials in Newborns

6.8K
Several important points for obtaining high-quality reliable visual evoked potentials (VEPs) in newborns while minimizing variability and the risk of misleading prognoses are...
6.8K
Measuring Psoriasis Severity at Home02:28

Measuring Psoriasis Severity at Home

776
This paper details the creation of a Psoriasis Thickness Reference Card, enabling psoriasis patients to self-assess their psoriasis plaque thickness without the need for physician assistance. Additionally, the precision of self-assessment for psoriasis redness and scaliness by employing reference images depicting various skin tones is...
776
Establishing a Severe Corneal Inflammation Model in Rats Based on Corneal Epithelium Curettage Combined with Corneal Sutures04:48

Establishing a Severe Corneal Inflammation Model in Rats Based on Corneal Epithelium Curettage Combined with Corneal Sutures

788
We developed a rat model of severe corneal inflammation through corneal epithelium curettage combined with corneal sutures. The study evaluated corneal inflammation patterns, epithelial proliferation, and changes in limbal stem cells under inflammatory...
788
Isolation and Differentiation of Primary White and Brown Preadipocytes from Newborn Mice09:00

Isolation and Differentiation of Primary White and Brown Preadipocytes from Newborn Mice

13.4K
This report describes a protocol for the simultaneous isolation of primary brown and white preadipocytes from newborn mice. Isolated cells can be grown in culture and induced to differentiate into fully mature white and brown adipocytes. The method enables genetic, molecular, and functional characterization of primary fat cells in...
13.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Ferumoxytol-enhanced Ultrashort Echo Time MRI of Cardiovascular Stents in Patients with Congenital Heart Disease: A Feasibility Study.

Radiology. Cardiothoracic imaging·2026
Same author

Coronary computed Tomography-Based surveillance for coronary allograft vasculopathy in pediatric heart transplant recipients: The role of CT-derived fractional flow reserve.

Journal of cardiovascular computed tomography·2025
Same author

Electronic Dose Monitoring Feedback (EMF) with Youth Living with HIV: Qualitative Exploration of Reactions to Viewing Dosing Calendars.

AIDS and behavior·2025
Same author

Vaccine Acceptance Among Youth Living With HIV in Detroit.

Clinical pediatrics·2025
Same author

Ventricular function assessment using an ultrafast spoiled gradient echo sequence with an intravascular blood pool contrast agent in pediatric patients.

PloS one·2025
Same author

Untrained Network for Super-resolution for Non-contrast-enhanced Wholeheart MRI Acquired using Cardiac-triggered REACT (SRNN-REACT).

Current medical imaging·2024

Related Experiment Video

Updated: Jan 20, 2026

Preparing a Mice Model of Severe Acute Pancreatitis via a Combination of Caerulein and Lipopolysaccharide Intraperitoneal Injection
07:38

Preparing a Mice Model of Severe Acute Pancreatitis via a Combination of Caerulein and Lipopolysaccharide Intraperitoneal Injection

Published on: May 10, 2024

1.2K

Newborn Screening for Severe Combined Immunodeficiency.

Mohammed Taki1, Tayaba Miah1, Elizabeth Secord2

  • 1Department of Pediatrics, Children's Hospital of Michigan, 3901 Beaubien Street, Detroit, MI 48201, USA.

Pediatric Clinics of North America
|August 31, 2019
PubMed
Summary

The T-cell receptor excision circle (TREC) assay is a sensitive and cost-effective newborn screening tool for severe combined immunodeficiency (SCID). It detects SCID and other conditions causing low T-cell counts, guiding diagnosis and management.

Keywords:
Newborn screening (NBS)Severe combined immunodeficiency (SCID)T-cell lymphopeniaT-cell receptor excision circle (tREC)

More Related Videos

How to Obtain Reliable Visual Event-related Potentials in Newborns
07:39

How to Obtain Reliable Visual Event-related Potentials in Newborns

Published on: October 24, 2019

6.8K
Author Spotlight: Self-Assessment Protocol for Predicting Psoriatic Arthritis in Psoriasis Patients
02:28

Author Spotlight: Self-Assessment Protocol for Predicting Psoriatic Arthritis in Psoriasis Patients

Published on: March 1, 2024

776

Related Experiment Videos

Last Updated: Jan 20, 2026

Preparing a Mice Model of Severe Acute Pancreatitis via a Combination of Caerulein and Lipopolysaccharide Intraperitoneal Injection
07:38

Preparing a Mice Model of Severe Acute Pancreatitis via a Combination of Caerulein and Lipopolysaccharide Intraperitoneal Injection

Published on: May 10, 2024

1.2K
How to Obtain Reliable Visual Event-related Potentials in Newborns
07:39

How to Obtain Reliable Visual Event-related Potentials in Newborns

Published on: October 24, 2019

6.8K
Author Spotlight: Self-Assessment Protocol for Predicting Psoriatic Arthritis in Psoriasis Patients
02:28

Author Spotlight: Self-Assessment Protocol for Predicting Psoriatic Arthritis in Psoriasis Patients

Published on: March 1, 2024

776

Area of Science:

  • Immunology
  • Genetics
  • Pediatrics

Background:

  • Severe combined immunodeficiency (SCID) is a group of rare genetic disorders characterized by profound defects in T-cell immunity.
  • Newborn screening is crucial for early detection and intervention to prevent life-threatening infections in infants with SCID.
  • The T-cell receptor excision circle (TREC) assay has emerged as a primary screening method for SCID.

Purpose of the Study:

  • To review the historical development and current applications of the TREC assay in newborn screening for SCID.
  • To elaborate on the different subtypes of SCID and the limitations associated with the TREC assay.
  • To discuss the diagnostic and management strategies for infants identified with SCID through newborn screening.

Main Methods:

  • Review of existing literature on SCID, newborn screening, and the TREC assay.
  • Analysis of the sensitivity, specificity, and cost-effectiveness of the TREC assay for SCID detection.
  • Discussion of clinical guidelines for follow-up testing and management of positive screens.

Main Results:

  • The TREC assay is a highly sensitive and cost-efficient method for SCID newborn screening.
  • The assay can detect typical SCID, leaky SCID, and other conditions associated with low naive T-cell counts.
  • Early detection via TREC assay facilitates timely diagnosis and improves outcomes for affected infants.

Conclusions:

  • The TREC assay is an effective tool for widespread newborn screening of SCID.
  • Understanding TREC assay limitations and SCID subtypes is essential for accurate diagnosis.
  • Prompt diagnostic evaluation and management are critical for infants with positive SCID screens to improve survival and long-term health.