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Related Concept Videos

A Technique to Assess the Immune Response against an Antigen by Inducing Skin Inflammation02:49

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Related Experiment Video

Updated: Jan 20, 2026

A Technique to Assess the Immune Response against an Antigen by Inducing Skin Inflammation
02:49

A Technique to Assess the Immune Response against an Antigen by Inducing Skin Inflammation

559

Skin immunization with third-generation hepatitis B surface antigen using microneedles.

Thuy Trang Nguyen1, Jung-Ah Choi2, Ji Seok Kim1

  • 1Department of BioNano Technology, Gachon BioNano Research Institute, Gachon University, Seongnam, Republic of Korea.

Vaccine
|August 31, 2019
PubMed
Summary
This summary is machine-generated.

This study demonstrates that microneedle delivery of L-HBsAg vaccine without adjuvant offers superior efficacy and stability compared to traditional intramuscular injections. This novel approach enhances hepatitis B vaccine delivery for potentially therapeutic treatments.

Keywords:
AdjuvantMicroneedlesSkin immunizationThird-generation hepatitis B surface antigen (L-HBsAg)

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Area of Science:

  • Vaccinology
  • Biomaterials Science
  • Drug Delivery Systems

Background:

  • Hepatitis B virus (HBV) infection remains a significant global health concern.
  • L-HBsAg, a third-generation hepatitis vaccine, shows potential for therapeutic treatment in non-responders.
  • Conventional intramuscular (IM) immunization often requires adjuvants and may have limitations in efficacy and patient compliance.

Purpose of the Study:

  • To evaluate the efficacy and stability of intradermal (ID) immunization using L-HBsAg-coated microneedles (MN) without an adjuvant.
  • To compare ID microneedle delivery with conventional L-HBsAg intramuscular immunization using aluminum hydroxide adjuvant (L-HBsAg-AL-IM).
  • To assess the impact of trehalose as a stabilizer on L-HBsAg-MN formulation stability.

Main Methods:

  • L-HBsAg was dip-coated onto polylactic acid (PLA) microneedles (800 μm length).
  • In vitro studies using porcine skin evaluated delivery efficiency and administration time.
  • Formulation stability was assessed against gamma-ray sterilization, storage at 40°C, and freeze-thaw cycles, with trehalose as a stabilizer.
  • Antibody titers were compared between L-HBsAg-MN-Tre and L-HBsAg-AL-IM groups at 28 days.

Main Results:

  • The L-HBsAg formulation was successfully coated on the microneedle tips and delivered within 30 minutes in vitro.
  • Trehalose significantly enhanced the stability of L-HBsAg-MN, with 15% trehalose providing stability for 7 days at 40°C and improved freeze-thaw resistance.
  • L-HBsAg-MN-Tre demonstrated a higher antibody titer at 28 days compared to L-HBsAg-AL-IM.
  • ID administration of L-HBsAg-MN-Tre showed superior efficacy and stability over L-HBsAg-AL-IM.

Conclusions:

  • Intradermal delivery of L-HBsAg using microneedles with trehalose as a stabilizer (L-HBsAg-MN-Tre) offers improved efficacy and stability.
  • This adjuvant-free microneedle system presents a promising alternative for hepatitis B vaccination, enhancing convenience and safety, particularly for pediatric applications.
  • L-HBsAg-MN-Tre demonstrates potential for therapeutic treatment of non-responders and improved vaccine administration.