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A Novel In Vitro Wound Healing Assay to Evaluate Cell Migration08:55

A Novel In Vitro Wound Healing Assay to Evaluate Cell Migration

Here, we present a protocol to evaluate the effect of peptides on the migration of bronchial epithelial cells. This method allows for the rapid and highly reproducible obtainment of quantitative data on the speed of cell migration and wound...
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Murine Model of Wound Healing05:39

Murine Model of Wound Healing

A murine model of cutaneous wound healing that can be used to assess therapeutic compounds in physiological and pathophysiological...
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An Epithelial Abrasion Model for Studying Corneal Wound Healing04:45

An Epithelial Abrasion Model for Studying Corneal Wound Healing

Here, a protocol for creating a central corneal epithelial abrasion wound in the mouse using a trephine and a blunt golf club spud is described. This corneal wound healing model is highly reproducible and is now being used to evaluate compromised corneal wound healing in the context of...
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Mongolian Gerbils as an Animal Model of Wound Healing07:54

Mongolian Gerbils as an Animal Model of Wound Healing

This article describes a new animal model developed to study the anatomy and histology of the cornea and its healing processes. This new animal model uses the Mongolian gerbil, which has a cornea with many similarities to the human...
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Murine Excisional Wound Healing Model and Histological Morphometric Wound Analysis06:36

Murine Excisional Wound Healing Model and Histological Morphometric Wound Analysis

This protocol describes how to generate bilateral, full-thickness excisional wounds in mice and how to subsequently monitor, harvest, and prepare the wounds for morphometric analysis. Included is an in-depth description of how to use serial histological sections to define, precisely quantify and detect morphometric...
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Visualizing Cytoplasmic Flow During Single-cell Wound Healing in Stentor coeruleus08:09

Visualizing Cytoplasmic Flow During Single-cell Wound Healing in Stentor coeruleus

The giant ciliate Stentor coeruleus is a classical system for studying regeneration and wound healing in single cells. By imaging Stentor cells simultaneously at low and high magnification it is possible to measure cytoplasmic flows before, during, and after...
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