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Related Concept Videos

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Related Experiment Video

Updated: Jan 20, 2026

An Orthotopic Mouse Model of Anaplastic Thyroid Carcinoma
07:01

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Long Non-coding RNA Expression in Anaplastic Thyroid Carcinomas.

Yanping Wang1, Heather Hardin1, Ying-Hsia Chu1

  • 1Department of Pathology and Laboratory Medicine, University of Wisconsin School of Medicine and Public Health, Madison, WI, 53792, USA.

Endocrine Pathology
|August 31, 2019
PubMed
Summary
This summary is machine-generated.

Long non-coding RNAs (lncRNAs) like PCA3 and HAM-1 are upregulated in thyroid tumors, indicating their role in cancer progression. These findings suggest potential oncogenic functions for these lncRNAs in thyroid carcinogenesis.

Keywords:
Anaplastic thyroid carcinomaHAM-1In situ hybridizationLong non-coding RNAPCA3PCR arrayPapillary thyroid carcinomaRT-PCRThyroid carcinoma

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Area of Science:

  • Molecular Biology
  • Oncology
  • Genetics

Background:

  • Long non-coding RNAs (lncRNAs) are involved in gene regulation, epithelial-mesenchymal transition, and carcinogenesis.
  • The role of lncRNAs in thyroid cancer progression, particularly in papillary thyroid carcinoma (PTC) and anaplastic thyroid carcinoma (ATC), requires further investigation.

Purpose of the Study:

  • To identify specific lncRNAs that are upregulated during thyroid tumor progression from normal thyroid tissue to PTC and ATC.
  • To investigate the expression patterns of prostate cancer antigen 3 (PCA3) and HOX antisense intergenic RNA myeloid 1 (HAM-1) in various thyroid tumor types.

Main Methods:

  • Utilized an RT2 Profiler PCR Array Human Cancer Pathway Finder to screen for differentially expressed lncRNAs in ATC versus PTC.
  • Employed in situ hybridization (ISH) on a thyroid tissue microarray (TMA) and quantitative real-time PCR (qRT-PCR) on formalin-fixed paraffin-embedded (FFPE) tissues and cell lines to analyze PCA3 and HAM-1 expression.
  • Investigated the effect of TGF-β treatment on PCA3 expression in cultured thyroid carcinoma cell lines.

Main Results:

  • Identified 11 upregulated and 7 downregulated lncRNAs in ATCs compared to PTCs.
  • Demonstrated increased expression of PCA3 and HAM-1 in ATCs and PTCs compared to normal thyroid tissue via TMA analysis.
  • Confirmed elevated expression of PCA3 and HAM-1 in ATCs compared to normal thyroid and PTCs through qRT-PCR, with highest levels observed in ATC cell lines.
  • Observed increased PCA3 expression in PTC and ATC cell lines upon TGF-β treatment.

Conclusions:

  • The lncRNAs PCA3 and HAM-1 are significantly upregulated during thyroid tumor development and progression.
  • These upregulated lncRNAs, PCA3 and HAM-1, may function as oncogenes in thyroid carcinogenesis.
  • Further research into the specific mechanisms of PCA3 and HAM-1 in thyroid cancer is warranted.