Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Swine Model of Biofilm Infection and Invisible Wounds07:16

Swine Model of Biofilm Infection and Invisible Wounds

2.4K
Chronic wounds that are resistant to antibiotics are a major threat to the healthcare system. Biofilm infections are stubborn and hostile and can cause deficient functional wound closure. We report a clinically relevant swine model of biofilm-infected full-thickness chronic wounds. This model is powerful for mechanistic studies as well as for testing...
2.4K
Quantification, Viability Assessment, and Visualization Strategies for Acinetobacter Biofilms07:41

Quantification, Viability Assessment, and Visualization Strategies for Acinetobacter Biofilms

4.4K
This protocol describes the preparation of the inoculum, the biofilm quantification on microtiter plates using crystal violet dye, the viable count in biofilms, and the visualization of biofilms of...
4.4K
Intracranial Subarachnoidal Route of Infection for Investigating Roles of Streptococcus suis Biofilms in Meningitis in a Mouse Infection Model07:26

Intracranial Subarachnoidal Route of Infection for Investigating Roles of Streptococcus suis Biofilms in Meningitis in a Mouse Infection Model

7.7K
Here, we describe the intracranial subarachnoidal route of infection in mice to study roles of biofilms in Streptococcus suis meningitis. This infection model is also suitable for studying the pathogenesis of other bacterial meningitis and the efficacy of new drugs against bacterial...
7.7K
Cystic Fibrosis Aggregate Biofilm Model to Study Infection-relevant Gene Expression08:58

Cystic Fibrosis Aggregate Biofilm Model to Study Infection-relevant Gene Expression

1.0K
This study outlines a framework for developing an aggregate polymicrobial biofilm model and optimizing an RNA extraction protocol to assess Pseudomonas aeruginosa gene expression reflective of the cystic fibrosis lung environment. Applications include evaluating antimicrobial effects and studying antibiotic alternatives under conditions relevant to cystic...
1.0K
Harvesting and Disaggregation: An Overlooked Step in Biofilm Methods Research13:25

Harvesting and Disaggregation: An Overlooked Step in Biofilm Methods Research

4.7K
This paper details methods that demonstrate three common biofilm harvesting and disaggregation techniques on two surface types, ruggedness testing of a harvesting method and minimum information to consider when choosing and optimizing harvesting and disaggregation techniques to increase...
4.7K
Treatment Strategies for Psychological Disorders01:24

Treatment Strategies for Psychological Disorders

739
Treatment approaches for psychological disorders fall into three main categories: psychological, biological, and sociocultural. Each approach targets different aspects of mental health, requiring varying levels of education and training.
Psychological therapies focus on modifying emotions, thoughts, and behaviors through talking, interpreting, listening, rewarding, challenging, and modeling. Clinical psychologists, counselors, and social workers commonly practice psychotherapy. Clinical...
739

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Modulation of the biological response to surfaces through the controlled deposition of 3D polymeric surfactants.

Journal of materials chemistry. B·2025
Same author

Innate immune mechanisms promote human response to Acinetobacter baumannii infection.

European journal of immunology·2024
Same author

Clostridioides difficile Biofilm.

Advances in experimental medicine and biology·2024
Same author

The Multifunctional Role of Poloxamer P338 as a Biofilm Disrupter and Antibiotic Enhancer: A Small Step forward against the Big Trouble of Catheter-Associated <i>Escherichia coli</i> Urinary Tract Infections.

Microorganisms·2022
Same author

An intercellular transfer of telomeres rescues T cells from senescence and promotes long-term immunological memory.

Nature cell biology·2022
Same author

Synthesis of Novel Hyaluronic Acid Sulfonated Hydrogels Using Safe Reactants: A Chemical and Biological Characterization.

Gels (Basel, Switzerland)·2022

Related Experiment Video

Updated: Jan 20, 2026

Author Spotlight: Studying Host-Microbe Interactions in Wound Biofilm Formation
07:16

Author Spotlight: Studying Host-Microbe Interactions in Wound Biofilm Formation

Published on: June 16, 2023

2.4K

Novel Treatment Strategies for Biofilm-Based Infections.

Claudia Vuotto1, Gianfranco Donelli2

  • 1Microbial Biofilm Laboratory, IRCCS Fondazione Santa Lucia, Via Ardeatina 306, Rome, Italy.

Drugs
|August 31, 2019
PubMed
Summary

Biofilms, communities of microbes, are highly resistant to antibiotics, leading to treatment failures. This review explores new strategies to combat biofilms without promoting further antibiotic resistance.

More Related Videos

Author Spotlight: A Comprehensive Protocol for Acinetobacter Biofilm Quantification, Assessment, and Visualization
07:41

Author Spotlight: A Comprehensive Protocol for Acinetobacter Biofilm Quantification, Assessment, and Visualization

Published on: August 4, 2023

4.4K
Intracranial Subarachnoidal Route of Infection for Investigating Roles of Streptococcus suis Biofilms in Meningitis in a Mouse Infection Model
07:26

Intracranial Subarachnoidal Route of Infection for Investigating Roles of Streptococcus suis Biofilms in Meningitis in a Mouse Infection Model

Published on: July 1, 2018

7.7K

Related Experiment Videos

Last Updated: Jan 20, 2026

Author Spotlight: Studying Host-Microbe Interactions in Wound Biofilm Formation
07:16

Author Spotlight: Studying Host-Microbe Interactions in Wound Biofilm Formation

Published on: June 16, 2023

2.4K
Author Spotlight: A Comprehensive Protocol for Acinetobacter Biofilm Quantification, Assessment, and Visualization
07:41

Author Spotlight: A Comprehensive Protocol for Acinetobacter Biofilm Quantification, Assessment, and Visualization

Published on: August 4, 2023

4.4K
Intracranial Subarachnoidal Route of Infection for Investigating Roles of Streptococcus suis Biofilms in Meningitis in a Mouse Infection Model
07:26

Intracranial Subarachnoidal Route of Infection for Investigating Roles of Streptococcus suis Biofilms in Meningitis in a Mouse Infection Model

Published on: July 1, 2018

7.7K

Area of Science:

  • Microbiology
  • Infectious Diseases
  • Drug Discovery

Background:

  • Microbial biofilms exhibit significantly higher antimicrobial tolerance compared to planktonic cells.
  • This enhanced tolerance is attributed to diffusion barriers and the spread of resistance genes within biofilms.
  • Biofilm-related infections are a major cause of chronic diseases and antibiotic treatment failures.

Purpose of the Study:

  • To review and evaluate emerging anti-biofilm strategies.
  • To identify safe, effective, and rapid therapeutic approaches against biofilms.
  • To focus on methods that do not exert evolutionary pressure for microbial resistance.

Main Methods:

  • Literature review of recent studies on biofilm formation and dispersal.
  • Evaluation of molecules and tools targeting biofilm development.
  • Assessment of strategies for dispersing pre-formed biofilms.

Main Results:

  • Several promising tools and molecules have been identified.
  • These agents modulate biofilm formation or disperse established biofilms.
  • Key strategies aim to overcome antimicrobial tolerance without driving resistance.

Conclusions:

  • Developing novel anti-biofilm strategies is crucial for combating chronic infections.
  • Targeting biofilm-specific mechanisms offers a promising alternative to conventional antibiotics.
  • Future research should focus on clinically translatable, resistance-free anti-biofilm therapies.