Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

In Vivo Targeting of Neural Progenitor Cells in Ferret Neocortex by In Utero Electroporation07:03

In Vivo Targeting of Neural Progenitor Cells in Ferret Neocortex by In Utero Electroporation

6.4K
Presented here is a protocol to perform genetic manipulation in the embryonic ferret brain using in utero electroporation. This method allows for targeting of neural progenitor cells in the neocortex in...
6.4K
Ex Vivo Culture of Chick Cerebellar Slices and Spatially Targeted Electroporation of Granule Cell Precursors10:02

Ex Vivo Culture of Chick Cerebellar Slices and Spatially Targeted Electroporation of Granule Cell Precursors

9.7K
The cerebellar external granule layer is the site of the largest transit amplification in the developing brain. Here, we present a protocol to target genetic modification to this layer at the peak of proliferation using ex vivo electroporation and culture of cerebellar slices from embryonic Day 14 chick...
9.7K
Transfection of Mouse Retinal Ganglion Cells by in vivo Electroporation05:26

Transfection of Mouse Retinal Ganglion Cells by in vivo Electroporation

16.0K
We demonstrate an in vivo electroporation protocol for transfecting single or small clusters of retinal ganglion cells (RGCs) and other retinal cell types in postnatal mice over a wide range of ages. The ability to label and genetically manipulate postnatal RGCs in vivo is a powerful tool for developmental...
16.0K
Targeting Olfactory Bulb Neurons Using Combined In Vivo Electroporation and Gal4-Based Enhancer Trap Zebrafish Lines08:18

Targeting Olfactory Bulb Neurons Using Combined In Vivo Electroporation and Gal4-Based Enhancer Trap Zebrafish Lines

12.6K
The temporal and spatial resolution of genetic manipulations determines the spectrum of biological phenomena that they can perturb. Here we use temporally and spatially discrete in vivo electroporation, combined with transgenic lines of zebrafish, to induce expression of a GFP transgene specifically in neurons of the developing olfactory...
12.6K
In vivo Electroporation of Developing Mouse Retina05:53

In vivo Electroporation of Developing Mouse Retina

21.5K
A method for the incorporation of plasmid DNA into murine retinal cells for the purpose of performing either gain- or loss of function studies in vivo is presented. This method capitalizes on the transient increase in permeability of cell plasma membranes induced by the application of an external electrical...
21.5K
In Vivo Microinjection and Electroporation of Mouse Testis08:39

In Vivo Microinjection and Electroporation of Mouse Testis

29.1K
This article describes microinjection and electroporation of mouse testis in vivo as a transfection technique for testicular mouse cells to study unique processes of spermatogenesis. The presented protocol involves steps of glass capillary preparation, microinjection via the efferent duct, and transfection by...
29.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Multiparametric Characterization of Tough Clots Following Stroke Thrombectomy.

Clinical neuroradiology·2026
Same author

A clinically integrated, frameless human Neuropixels workflow.

medRxiv : the preprint server for health sciences·2026
Same author

Sine spin flat-detector CT for enhanced brain parenchyma visualization: an intraindividual comparison on a latest-generation angiography system.

Neuroradiology·2026
Same author

High-resolution ultrasound vs. MR neurography in upper extremity neuropathies: exploratory analysis of perceived additional diagnostic value in routine clinical practice.

European radiology·2026
Same author

Structure-based design of pyrazole derivatives targeting the human Cyclophilin D binding site.

International journal of biological macromolecules·2026
Same author

Critical point drying of brain tissue for X-ray phase-contrast imaging.

Journal of synchrotron radiation·2026

Related Experiment Video

Updated: Jan 20, 2026

In Vivo Targeting of Neural Progenitor Cells in Ferret Neocortex by In Utero Electroporation
07:03

In Vivo Targeting of Neural Progenitor Cells in Ferret Neocortex by In Utero Electroporation

Published on: May 6, 2020

6.4K

Targeted In Vivo Electroporation Using Nanoengineered Microelectrodes.

Daniel Schwarz1,2,3, Andreas T Schaefer4,5,6,7

  • 1Behavioural Neurophysiology, Max-Planck-Institute for Medical Research, Heidelberg, Germany.

Methods in Molecular Biology (Clifton, N.J.)
|August 31, 2019
PubMed
Summary
This summary is machine-generated.

Nanoengineered electroporation microelectrodes (NEMs) improve targeted electroporation in living tissues. These novel electrodes offer a more even electric field distribution, expanding the possibilities for cellular manipulation without causing damage.

Keywords:
ElectroporationIn vivoMicroelectrodesNanoengineering

More Related Videos

Ex Vivo Culture of Chick Cerebellar Slices and Spatially Targeted Electroporation of Granule Cell Precursors
10:02

Ex Vivo Culture of Chick Cerebellar Slices and Spatially Targeted Electroporation of Granule Cell Precursors

Published on: December 14, 2015

9.7K
Transfection of Mouse Retinal Ganglion Cells by in vivo Electroporation
05:26

Transfection of Mouse Retinal Ganglion Cells by in vivo Electroporation

Published on: April 17, 2011

16.0K

Related Experiment Videos

Last Updated: Jan 20, 2026

In Vivo Targeting of Neural Progenitor Cells in Ferret Neocortex by In Utero Electroporation
07:03

In Vivo Targeting of Neural Progenitor Cells in Ferret Neocortex by In Utero Electroporation

Published on: May 6, 2020

6.4K
Ex Vivo Culture of Chick Cerebellar Slices and Spatially Targeted Electroporation of Granule Cell Precursors
10:02

Ex Vivo Culture of Chick Cerebellar Slices and Spatially Targeted Electroporation of Granule Cell Precursors

Published on: December 14, 2015

9.7K
Transfection of Mouse Retinal Ganglion Cells by in vivo Electroporation
05:26

Transfection of Mouse Retinal Ganglion Cells by in vivo Electroporation

Published on: April 17, 2011

16.0K

Area of Science:

  • Biotechnology
  • Cell Biology
  • Nanotechnology

Background:

  • Targeted electroporation using glass microelectrodes is a key technique for cell manipulation in living tissues.
  • A major limitation is the narrow range for reversible electroporation due to focal electric field distribution, especially for larger volumes.
  • This restricts precise cellular manipulation and genetic modification in complex biological systems.

Purpose of the Study:

  • To develop nanoengineered electroporation microelectrodes (NEMs) for improved targeted electroporation.
  • To overcome the limitations of conventional glass microelectrodes regarding electric field distribution.
  • To enable precise and safe manipulation of larger cellular volumes in living tissues.

Main Methods:

  • Fabrication of glass microelectrodes using nanotechnological methods to create nanoengineered features.
  • Characterization of the electric field distribution around the modified microelectrode tips.
  • Testing the efficacy and safety of NEMs for reversible electroporation in cellular models.

Main Results:

  • NEMs demonstrate a more even electric field distribution around the pipette tip compared to conventional electrodes.
  • This improved distribution allows for a wider range of reversible electroporation parameters.
  • Successful application of NEMs for targeted electroporation of larger cellular volumes with reduced irreversible damage.

Conclusions:

  • Nanoengineered electroporation microelectrodes offer a practical advancement over traditional methods.
  • NEMs enhance the precision and safety of cellular manipulation via electroporation.
  • This technology holds significant potential for applications in cell biology, tissue engineering, and regenerative medicine.