Transformation of the Model Microalga Chlamydomonas reinhardtii Without Cell-Wall Removal

Takashi Yamano1, Hideya Fukuzawa2

  • 1Graduate School of Biostudies, Kyoto University, Kyoto, Japan.

Related Concept Videos

Reactivation of Demembranated Cell Models in Chlamydomonas reinhardtii03:37

Reactivation of Demembranated Cell Models in Chlamydomonas reinhardtii

The in vitro reactivation of motile cells is a crucial experiment in understanding the mechanisms of cell motility. The protocol describes reactivating the demembranated cell models of Chlamydomonas reinhardtii, a model organism to study...
2.5K
Observation of Photobehavior in Chlamydomonas reinhardtii03:54

Observation of Photobehavior in Chlamydomonas reinhardtii

Most swimming photoautotrophic organisms show photo-induced behavioral changes (photobehavior). The present protocol observes the said photobehavior in the model organism Chlamydomonas...
4.6K
High-Throughput Robotically Assisted Isolation of Temperature-sensitive Lethal Mutants in Chlamydomonas reinhardtii10:51

High-Throughput Robotically Assisted Isolation of Temperature-sensitive Lethal Mutants in Chlamydomonas reinhardtii

Temperature-sensitive (ts) lethal mutants are valuable tools to identify and analyze essential functions. Here we describe methods to generate and classify ts lethal mutants in high throughput.
10.3K
Mating and Tetrad Separation of Chlamydomonas reinhardtii for Genetic Analysis10:04

Mating and Tetrad Separation of Chlamydomonas reinhardtii for Genetic Analysis

Mating and tetrad separation are required for genetic analysis in Chlamydomonas reinhardtii. Here we demonstrate standard methods for gametogenesis, mating, zygote germination and tetrad dissection. This protocol consists of an easy-to-follow series of steps that will make genetic approaches amenable to scientists who are less familiar with...
20.7K
High-Throughput Metabolic Profiling for Model Refinements of Microalgae11:07

High-Throughput Metabolic Profiling for Model Refinements of Microalgae

This protocol demonstrates the use of a phenotype microarray (PM) technology platform to define metabolic requirements of Chlamydomonas reinhardtii, a green microalga, and refine an existing metabolic network...
4.2K
A Multi-Omics Extraction Method for the In-Depth Analysis of Synchronized Cultures of the Green Alga Chlamydomonas reinhardtii07:51

A Multi-Omics Extraction Method for the In-Depth Analysis of Synchronized Cultures of the Green Alga Chlamydomonas reinhardtii

System-wide analysis of multiple biomolecules is crucial to gain functional and mechanistic insights into biological processes. Hereby, an extensive protocol is described for high throughput extraction of lipids, metabolites, proteins and starch from a single sample harvested from synchronized Chlamydomonas...
8.1K