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Updated: Jan 20, 2026

Reactivation of Demembranated Cell Models in Chlamydomonas reinhardtii
Published on: May 6, 2022
Transformation of the Model Microalga Chlamydomonas reinhardtii Without Cell-Wall Removal
Takashi Yamano1, Hideya Fukuzawa2
1Graduate School of Biostudies, Kyoto University, Kyoto, Japan.
Abstract:
The green alga Chlamydomonas reinhardtii has been widely used to study many biological processes, including photosynthesis, flagellar motility, sexual reproduction, metabolism, and genetics. Here, we describe a step-by-step protocol of rapid and efficient transformation method for wild type cell-walled Chlamydomonas strains without cell-wall removal using a square electric pulses-generating electroporator. This method could be applied to the transformation of other industrially useful algae including diatom by optimizing the electric conditions.
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