Related Experiment Video

Updated: Jan 20, 2026

Bacterial Transformation: Electroporation
12:19

Bacterial Transformation: Electroporation

Published on: April 30, 2023

119.2K

Electroporation Transformation Protocol for Phaeodactylum tricornutum

Hanhua Hu1, Yufang Pan2

  • 1Key Laboratory of Algal Biology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, China. hanhuahu@ihb.ac.cn.

Methods in Molecular Biology (Clifton, N.J.)
|August 31, 2019
PubMed
Summary

We developed a high-efficiency genetic transformation method for Phaeodactylum tricornutum using electroporation. This protocol can aid in developing similar systems for other microalgae, advancing diatom research and applications.

Keywords:
DiatomsElectroporationPhaeodactylum tricornutumTransformation

More Related Videos

Preparation and Transformation of Bacteria Using High-Voltage Electroporation03:33

Preparation and Transformation of Bacteria Using High-Voltage Electroporation

Published on: October 30, 2025

298
Author Spotlight: Methods for Electroporation and Transformation Confirmation in Limosilactobacillus reuteri DSM2001611:04

Author Spotlight: Methods for Electroporation and Transformation Confirmation in Limosilactobacillus reuteri DSM20016

Published on: June 23, 2023

5.0K

Related Concept Videos

Bacterial Transformation: Electroporation12:19

Bacterial Transformation: Electroporation

The term “transformation” refers cellular ingestion of foreign DNA. In nature, transformation can occur in certain types of bacteria. In molecular biology, however, transformation is artificially induced through the creation of pores in the bacterial cell walls. Bacterial cells that are able to take up DNA from the environment are called competent cells. Electrocompetent cells can be produced in the laboratory and transformation of these cells can be achieve via the...
119.2K
Preparation and Transformation of Bacteria Using High-Voltage Electroporation03:33

Preparation and Transformation of Bacteria Using High-Voltage Electroporation

Source: Stefan, A., et al., The Multifaceted Benefits of Protein Co-expression in Escherichia coli. J. Vis. Exp. (2015)This video demonstrates the procedure for transforming Escherichia coli with a plasmid carrying an antibiotic resistance gene, including cell preparation, electroporation, recovery, and selection of...
298
Methods for Electroporation and Transformation Confirmation in Limosilactobacillus reuteri DSM2001611:04

Methods for Electroporation and Transformation Confirmation in Limosilactobacillus reuteri DSM20016

Here, we present protocols for working with Limosilactobacillus reuteri DSM20016, detailing growth, plasmid transformation, colony PCR, fluorescent reporter protein measurement, and limited plasmid mini-prep, as well as common issues and troubleshooting. These protocols allow the measurement of reporter proteins in DSM20016, or confirmation via colony PCR if no reporter is involved.
5.0K
Electroporation of Mycobacteria11:57

Electroporation of Mycobacteria

Mycobacterial pathogenic strategies remain poorly understood. The slow growth rate of most species, the impenetrable nature of the cell-wall, and the hazards of working with pathogens make mycobacteria difficult to study and are largely responsible for our poor understanding of these organisms. In this video we will demonstrate the technique of electroporation, which involves subjecting cells to a brief high electrical impulse to allow the entry of DNA. It is the most widely used method for...
26.0K
An Electroporation Method to Transform Rickettsia spp. with a Fluorescent Protein-Expressing Shuttle Vector in Tick Cell Lines07:18

An Electroporation Method to Transform Rickettsia spp. with a Fluorescent Protein-Expressing Shuttle Vector in Tick Cell Lines

Electroporation is a rapid, broadly adopted method for introducing exogenous DNA into the genus Rickettsia. This protocol provides a useful electroporation method for the transformation of obligate intracellular bacteria in the genus Rickettsia.
2.4K
Universal and Efficient Electroporation Protocol for Genetic Engineering of Gastrointestinal Organoids07:19

Universal and Efficient Electroporation Protocol for Genetic Engineering of Gastrointestinal Organoids

This protocol describes an efficient electroporation method for the transfection of four different gastrointestinal organoid entities with larger plasmids (to the extent of 10 kB). It can be performed within one day and does not need extensive preparation or special, cost-intensive electroporation...
18.7K