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Updated: Dec 25, 2025

Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System

Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System

Published on: April 25, 2025

Update on the epidemiological typing methods for Acinetobacter baumannii.

Rayane Rafei¹, Marwan Osman¹, Fouad Dabboussi¹

¹Laboratoire Microbiologie Santé et Environnement (LMSE), Doctoral School of Science and Technology, Faculty of Public Health, Lebanese University, Tripoli, Lebanon.

Future Microbiology

|August 31, 2019

Summary

This summary is machine-generated.

Acinetobacter baumannii typing methods are evolving from traditional techniques to whole genome sequencing. This shift offers advanced applications for tracking multidrug-resistant infections and improving hospital outbreak control.

Keywords:

CRISPR-based typing SNP-based approaches accessory genome gene-by-gene based approaches outbreak spectroscopic technologies traditional typing methods typing whole genome sequencing

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The Application of Open Searching-based Approaches for the Identification of Acinetobacter baumannii O-linked Glycopeptides

The Application of Open Searching-based Approaches for the Identification of Acinetobacter baumannii O-linked Glycopeptides

Published on: November 2, 2021

Area of Science:

Microbiology
Infectious Diseases
Genomics

Background:

Acinetobacter baumannii is a significant pathogen known for causing outbreaks and acquiring multidrug resistance.
Effective typing methods are crucial for infection control and epidemiological surveillance in healthcare settings.

Purpose of the Study:

To review and update the landscape of Acinetobacter baumannii typing techniques.
To highlight the transition from traditional methods to advanced whole genome sequencing approaches.

Main Methods:

Review of established typing techniques for Acinetobacter baumannii.
Discussion of emerging methods, including clustered regularly interspaced short palindromic repeats (CRISPR) typing.
Emphasis on the applications and advantages of whole genome sequencing (WGS).

Main Results:

Traditional typing methods have historically aided in Acinetobacter baumannii outbreak investigations.
Newer methods like CRISPR typing offer alternative approaches.
Whole genome sequencing provides unprecedented resolution and novel applications for microbial typing.

Conclusions:

The evolution of typing methodologies, particularly WGS, is transforming the surveillance and control of Acinetobacter baumannii infections.
Advanced genomic approaches enhance the ability to manage multidrug-resistant strains and hospital-acquired outbreaks.