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Related Concept Videos

A Peptide Microarray Technology for Specificity Profiling of Antibodies05:21

A Peptide Microarray Technology for Specificity Profiling of Antibodies

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This video demonstrates a technique that applies peptide microarray technology to profile the specificity of antibodies recognizing post-translational modifications (PTMs) on histone peptides. A microarray slide containing immobilized fluorophore-conjugated histone peptides with known PTM combinations is treated with target PTM-specific antibodies. This process helps identify the antibody's specificity for the target and its potential cross-reactivity with non-target...
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Extracellular Protein Microarray Technology for High Throughput Detection of Low Affinity Receptor-Ligand Interactions06:01

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Here, we present a protocol to screen extracellular protein microarrays for identification of novel receptor-ligand interactions in high throughput. We also describe a method to enhance detection of transient protein-protein interactions by using protein-microbead...
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JUMPn: A Streamlined Application for Protein Co-Expression Clustering and Network Analysis in Proteomics07:28

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We present a systems biology tool JUMPn to perform and visualize network analysis for quantitative proteomics data, with a detailed protocol including data pre-processing, co-expression clustering, pathway enrichment, and protein-protein interaction network...
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Expression Profiling with Microarrays08:29

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Microarrays are important tools for profiling gene expression, and are based on complementary binding between probes that are attached to glass chips and nucleic acids derived from samples. Using these arrays, scientists can simultaneously evaluate the expression of thousands of genes. In addition, the expression profiles of different cells or tissue types can be compared, allowing researchers to deduce how the expression of different genes change during biological processes, and thus gain...
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Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions08:07

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Updated: Jan 20, 2026

A Peptide Microarray Technology for Specificity Profiling of Antibodies
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Proteome microarray technology and application: higher, wider, and deeper.

Huan Qi1, Fei Wang2, Sheng-Ce Tao1

  • 1Shanghai Center for Systems Biomedicine, Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Jiao Tong University , Shanghai , China.

Expert Review of Proteomics
|August 31, 2019
PubMed
Summary
This summary is machine-generated.

Functional proteome microarrays offer powerful high-throughput analysis for biological and clinical research. Future advancements promise expanded applications and deeper insights from these versatile tools.

Keywords:
Protein microarrayantibody microarrayproteome microarray

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Extracellular Protein Microarray Technology for High Throughput Detection of Low Affinity Receptor-Ligand Interactions
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Last Updated: Jan 20, 2026

A Peptide Microarray Technology for Specificity Profiling of Antibodies
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Extracellular Protein Microarray Technology for High Throughput Detection of Low Affinity Receptor-Ligand Interactions
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JUMPn: A Streamlined Application for Protein Co-Expression Clustering and Network Analysis in Proteomics
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Area of Science:

  • Proteomics
  • Biotechnology
  • Bioinformatics

Background:

  • Protein microarrays are miniaturized, sensitive platforms for parallel analysis.
  • Functional proteome microarrays are key tools in biological and clinical research.
  • High-throughput analysis reduces reagent and sample consumption.

Purpose of the Study:

  • To review recent advancements in functional proteome microarray construction and data interpretation.
  • To summarize proteomic-scale applications of protein microarray technology.
  • To address current limitations and future directions in the field.

Main Methods:

  • Discussion of novel construction strategies like PAGES and M-NAPPA.
  • Integration of technologies such as VirD.
  • Utilization of the first protein microarray database for data interpretation.

Main Results:

  • Overview of recently developed proteome microarrays.
  • Summary of proteomic-scale applications.
  • Identification of limitations and future research avenues.

Conclusions:

  • Proteome microarrays are indispensable for basic and clinical research.
  • Expectations for improved microarray designs and broader species coverage.
  • Anticipation of increased feature density, expanded applications, and enhanced data depth.