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Updated: Jan 20, 2026

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Deciphering Melatonin-Stabilized Phase Separation in Phospholipid Bilayers.

Dima Bolmatov, William T McClintic, Graham Taylor

    Langmuir : the ACS Journal of Surfaces and Colloids
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    Summary
    This summary is machine-generated.

    Melatonin, a brain hormone, stabilizes lipid rafts in model cell membranes. This hormone enhances the coexistence of liquid-ordered and liquid-disordered phases across a wider temperature range.

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    Area of Science:

    • Biochemistry
    • Biophysics
    • Cell Biology

    Background:

    • Lipid bilayers form cell membranes, enabling vital functions like cell recognition and signal transduction.
    • Lipid rafts, phase-separated membrane regions, are crucial sites for many biological processes.
    • Understanding membrane heterogeneity is key to deciphering cellular mechanisms.

    Purpose of the Study:

    • To investigate the effect of melatonin on phase-separated model membranes.
    • To determine if melatonin stabilizes lipid raft domains.
    • To explore melatonin's role in membrane fluidity and organization.

    Main Methods:

    • Utilized small angle neutron scattering (SANS) to analyze liposome structure.
    • Employed confocal fluorescence microscopy to visualize membrane domains.
    • Applied differential scanning calorimetry (DSC) to study phase transitions.
    • Developed a Landau-Brazovskii model for detecting nanoscopic domains.

    Main Results:

    • Melatonin demonstrated a stabilizing effect on liquid-ordered/liquid-disordered phase coexistence in model membranes.
    • This stabilization occurred over an extended temperature range.
    • The effect was observed in both nanometer- and micrometer-sized liposomes.
    • A novel model was developed to detect nanoscopic membrane heterogeneities.

    Conclusions:

    • Melatonin acts as a stabilizer for phase-separated lipid domains in model plasma membranes.
    • These findings suggest a role for melatonin in regulating membrane properties.
    • The developed Landau-Brazovskii model offers a new tool for studying nanoscale membrane organization.