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Related Concept Videos

Genome Editing08:31

Genome Editing

56.0K
A well-established technique for modifying specific sequences in the genome is gene targeting by homologous recombination, but this method can be laborious and only works in certain organisms. Recent advances have led to the development of “genome editing”, which works by inducing double-strand breaks in DNA using engineered nuclease enzymes guided to target genomic sites by either proteins or RNAs that recognize specific sequences. When a cell attempts to repair this damage,...
56.0K
CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

1.8K
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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Microinjection for Transgenesis and Genome Editing in Threespine Sticklebacks08:51

Microinjection for Transgenesis and Genome Editing in Threespine Sticklebacks

14.6K
Transgenic manipulations and genome editing are critical for functionally testing the roles of genes and cis-regulatory elements. Here a detailed microinjection protocol for the generation of genomic modifications (including Tol2-mediated fluorescent reporter transgene constructs, TALENs, and CRISPRs) is presented for the emergent model fish, the threespine...
14.6K
Breeding by Design for Functional Rice with Genome Editing Technologies09:43

Breeding by Design for Functional Rice with Genome Editing Technologies

3.4K
The protocol describes breeding resistant starch rice varieties by design using genome editing technologies in a precise, efficient, and technically simple...
3.4K
Lentiviral CRISPR/Cas9-Mediated Genome Editing for the Study of Hematopoietic Cells in Disease Models08:14

Lentiviral CRISPR/Cas9-Mediated Genome Editing for the Study of Hematopoietic Cells in Disease Models

12.9K
Described are protocols for the highly efficient genome editing of murine hematopoietic stem and progenitor cells (HSPC) by the CRISPR/Cas9 system to rapidly develop mouse model systems with hematopoietic system-specific gene modifications.
12.9K
Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms09:51

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

35.5K
Utilizing a preassembled Cas9 ribonucleoprotein complex (RNP) is a powerful method for precise, efficient genome editing. Here, we highlight its utility across a broad range of cells and organisms, including primary human cells and both classic and emerging model...
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Related Experiment Video

Updated: Jan 20, 2026

Genome Editing: ZFNs, TALENs, and CRISPR-cas9 System
08:31

Genome Editing: ZFNs, TALENs, and CRISPR-cas9 System

Published on: April 30, 2023

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Genome Editing and Hematologic Malignancy.

Brian T Emmer1, David Ginsburg2

  • 1Division of Hospital Medicine, Department of Internal Medicine and Life Sciences Institute, University of Michigan, Ann Arbor, Michigan 48109, USA;

Annual Review of Medicine
|August 31, 2019
PubMed
Summary
This summary is machine-generated.

Genome editing technologies offer promise for treating genetic diseases, but challenges remain for clinical application, especially in blood cancers. Further research is needed to overcome technical and conceptual barriers for safe and effective therapies.

Keywords:
CRISPRgene therapygenome editinghematologic malignancy

More Related Videos

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Microinjection for Transgenesis and Genome Editing in Threespine Sticklebacks

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Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits
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Microinjection for Transgenesis and Genome Editing in Threespine Sticklebacks
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Microinjection for Transgenesis and Genome Editing in Threespine Sticklebacks

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Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits
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Area of Science:

  • Genomics and Molecular Biology
  • Biotechnology
  • Translational Medicine

Background:

  • The genomic era has advanced disease understanding but lagged in translating discoveries into treatments.
  • Genome editing technologies represent a significant advancement with potential for direct genetic disease treatment.

Purpose of the Study:

  • To review the promise of genome editing therapies.
  • To highlight conceptual and technical barriers to clinical application.
  • To focus on hematologic malignancies as a key area.

Main Methods:

  • Review of current literature on genome editing technologies.
  • Analysis of potential therapeutic applications.
  • Examination of challenges in clinical translation.

Main Results:

  • Genome editing holds significant promise for treating genetic diseases.
  • Several conceptual and technical barriers impede clinical application.
  • Hematologic malignancies are a primary focus for potential therapies.

Conclusions:

  • Genome editing therapies offer a hopeful future for treating genetic disorders.
  • Overcoming barriers is crucial for successful clinical translation.
  • Further development is essential for applying these technologies to hematologic malignancies.