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Updated: Jan 20, 2026

Rapid and Cost-Effective RNA Extraction of Rat Pancreatic Tissue
Published on: September 19, 2020
An effective and rapid method for RNA preparation from non-conventional yeast species
Dong Wook Lee1, Chang Pyo Hong2, Hyun Ah Kang1
1Molecular Systems Biology Laboratory, Department of Life Science, Chung-Ang University, Seoul, 06974, South Korea.
Abstract:
The increased use of high-throughput RNA-based analysis has spurred the demand for rapid and simple preparation of high quality RNA. RNA preparation from non-conventional yeasts having diverse cell wall and morphological characteristics is often inefficient using current methods adapted for the model yeast, Saccharomyces cerevisiae. We report a simple RNA preparation method based on glass bead-mediated breakage in a formamide/EDTA solution. High quality RNA is generated within 15 min from various non-conventional yeasts species. The obtained RNA can be directly used for experimentation without further RNA purification and buffer exchange.
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