Effects of exercise in a cold environment on gene expression for mitochondrial biogenesis and mitophagy

Cold exposure during cycling and recovery enhances PGC-1α transcription, but aspects of mitophagy and a more intense cold exposure without recovery occurring in the cold have not been explored.

Determine the expression of genes related to mitochondrial biogenesis and mitophagy following an acute cycling bout at a temperature below freezing compared to that of room temperature.

Eleven male participants cycled at 65% W_max for 1 h at -2 °C and 20 °C and then recovered at room temperature for 6 h. A muscle biopsy was taken from the vastus lateralis before exercise, 3 h, and 6 h post-exercise for gene expression analysis.

Exercising heart rate and skin temperature were lower in the cold (p < 0.001; p = 0.004), while core temperature was higher (p = 0.016). Temperature had no effect on gene expression (p > 0.05). BNIP3 and BNIP3L mRNA were not influenced by exercise (p = 0.329; p 0.233). PGC-1α and VEGF were higher after cycling (p < 0.001), but the extent of PGC-1α upregulation was reduced 6 h post-exercise (p 0.006). TFAM increased 6 h post-exercise (p = 0.001). NRF2, ERRα, PINK1, and PARK2 decreased 3 h post-exercise (p 0.035; p = 0.005; p = 0.002; p = 0.001), but this downregulation was diminished after 6 h of recovery (p = 0.017; p 0.006; p = 0.043; p = 0.047). NRF1 was marginally attenuated with exercise (p = 0.001).

Exercise induced alterations in gene expression for mitochondrial biogenesis and mitophagy, but these effects were independent of temperature.