Related Experiment Video
Updated: Jan 20, 2026
Source and Route of Pyrrolizidine Alkaloid Contamination in Tea Samples
Published on: September 28, 2022
Melotenuines A-E, cytotoxic monoterpenoid indole alkaloids from Melodinus tenuicaudatus
Jian-Wen Liu1, Zong-Qing Huo2, Qian Zhao2
1Yunnan University of Traditional Chinese Medicine, Kunming 650500, PR China; State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, Yunnan, PR China.
Abstract:
Five new monoterpenoid indole alkaloids, melotenuines A-E (1-5), along with 18 known indole alkaloids, were isolated from the twigs and leaves of Melodinus tenuicaudatus. The structures of the new alkaloids were determined by a combination of MS, NMR and ECD analysis. Melotenuine A (1) represents the first example of aspidosperma-meloscandonine type bisindole alkaloids characterized by a methylene bridge between the two monomers, while melotenuine B (2) possessed a rare eburnamine-melsocandonine skeleton. All of the new indole alkaloids were evaluated for in vitro cytotoxicities against five human cancer cell lines. Among them, alkaloid 4 showed specific cytotoxicity against HL-60 cell line with IC50 value (5.15 ± 0.16 μM) comparable with that of positive control.
Related Concept Videos
06:04Source and Route of Pyrrolizidine Alkaloid Contamination in Tea Samples
09:36Applications of Liquid-Chromatography Tandem Mass Spectrometry in Natural Products Research: Tropane Alkaloids as a Case Study
09:46Accessing the Cytotoxicity and Cell Response to Biomaterials
08:22Cytotoxicity Assays with Zebrafish Cell Lines
08:56Detection of Regulated Ergot Alkaloids in Food Matrices by Liquid Chromatography-Trapped Ion Mobility Spectrometry-Time-of-Flight Mass Spectrometry
12:27Quantifying the Cytotoxicity of Staphylococcus aureus Against Human Polymorphonuclear Leukocytes