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Epigenetic Regulation

Epigenetic Regulation

Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.

Analysis of Transgenerational Epigenetic Inheritance in C. elegans Using a Fluorescent Reporter and Chromatin Immunoprecipitation (ChIP)

Analysis of Transgenerational Epigenetic Inheritance in C. elegans Using a Fluorescent Reporter and Chromatin Immunoprecipitation (ChIP)

This protocol describes an RNA interference and ChIP assay to study the epigenetic inheritance of RNAi-induced silencing and associated chromatin modifications in C. elegans.

An Overview of Epigenetics

An Overview of Epigenetics

Since the early days of genetics research, scientists have noted certain heritable phenotypic differences that are not due to differences in the nucleotide sequence of DNA. Current evidence suggests that these “epigenetic” phenomena might be controlled by a number of mechanisms, including the modification of DNA cytosine bases with methyl groups, the addition of various chemical groups to histone proteins, and the recruitment of protein factors to specific DNA sites via interactions...

Inheritance of Chromatin Structures

Inheritance of Chromatin Structures

Epigenetics is the study of inherited changes in a cell's phenotype without changing the DNA sequences. It provides a form of memory for the differential gene expression pattern to maintain cell lineage, position-effect variegation, dosage compensation, and maintenance of chromatin structures such as telomeres and centromeres. For example, the structure and location of the centromere on chromosomes are epigenetically inherited. Its functionality is not dictated or ensured by the underlying...

A Two-Step Strategy that Combines Epigenetic Modification and Biomechanical Cues to Generate Mammalian Pluripotent Cells

A Two-Step Strategy that Combines Epigenetic Modification and Biomechanical Cues to Generate Mammalian Pluripotent Cells

We here present a method that combines the use of chemical epigenetic erasing with mechanosensing-related cues to efficiently generate mammalian pluripotent cells, without the need of gene transfection or retroviral vectors. This strategy is, therefore, promising for translational medicine and represents a notable advancement in stem cell organoid...

Genomic Imprinting and Inheritance

Genomic Imprinting and Inheritance

Diploid organisms inherit genetic material through chromosomes from both parents. Copies of the same gene are known as alleles. In most cases, both alleles are simultaneously expressed and allow various cellular processes to function optimally. If one of the alleles is missing or mutated, the expression of the other allele can compensate; however, this is not true for all genes.
The expression of some genes depends on which parent passed the gene to the offspring, through a phenomenon known as...

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Related Experiment Video

Updated: Jan 20, 2026

Author Spotlight: RNAi Inheritance and ChIP in C. elegans

Author Spotlight: RNAi Inheritance and ChIP in C. elegans

Published on: May 5, 2023

Multigenerational epigenetic inheritance: One step forward, two generations back.

Jennifer J Tuscher¹, Jeremy J Day¹

¹Department of Neurobiology, Evelyn F. McKnight Brain Institute, University of Alabama at Birmingham, Birmingham, AL 35294, USA.

Neurobiology of Disease

|August 31, 2019

Summary

This summary is machine-generated.

Epigenetic modifications in the brain are crucial for its function and development. Emerging research suggests these epigenetic changes may be passed to offspring, but this inheritance is controversial and requires careful study.

Keywords:

DNA methylation Epigenetics Gene expression Heritability Histone modifications Non-coding RNA Transgenerational

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Last Updated: Jan 20, 2026

Author Spotlight: RNAi Inheritance and ChIP in C. elegans

Author Spotlight: RNAi Inheritance and ChIP in C. elegans

Published on: May 5, 2023

Epigenetic Regulation: DNA Methylation and Histone Modification

Epigenetic Regulation: DNA Methylation and Histone Modification

Epigenetics, lncRNAs and Epigenetic Code

Epigenetics, lncRNAs and Epigenetic Code

Published on: April 30, 2023

Area of Science:

Neuroscience
Epigenetics
Developmental Biology

Background:

Epigenetic modifications (DNA and histone) regulate gene expression in the developing and adult brain.
These modifications are vital for cellular diversity, experience-dependent learning, and are implicated in brain disorders.
Emerging evidence suggests potential for inter- and trans-generational epigenetic inheritance.

Purpose of the Study:

To review multigenerational epigenetic mechanisms in the central nervous system.
To explore cellular mechanisms of epigenetic information transfer and counteraction.
To discuss phenotypes, caveats, and technical limitations of multigenerational epigenetic effects.

Main Methods:

Literature review of existing research on multigenerational epigenetic mechanisms in the CNS.
Analysis of cellular mechanisms in germline and somatic cells.
Examination of phenotypes resulting from ancestral experiences and gene regulatory modifications.

Main Results:

Epigenetic patterns are critical for brain function and implicated in disease.
Potential for epigenetic information transfer across generations exists but is debated.
Multigenerational epigenetic effects require careful interpretation due to confounding factors.

Conclusions:

Understanding cellular events in epigenetic inheritance is incomplete.
The ability of epigenetic information to bypass embryonic reprogramming is controversial.
Studies on multigenerational epigenetic effects on the CNS need cautious interpretation and mechanistic disentanglement from genetic/environmental factors.