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A Fourier Transform Infrared Spectroscopy Technique to Study Peptide Self-Assembly
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Spectroscopic Characterization of Bovine, Avian and Johnin Purified Protein Derivative (PPD) with High-Throughput

Sara Corneli1, Laura Corte2, Luca Roscini3

  • 1Istituto Zooprofilattico Sperimentale dell'Umbria e delle Marche "Togo Rosati", Via Gaetano Salvemini 1, 06126 Perugia, Italy.

Pathogens (Basel, Switzerland)
|September 1, 2019
PubMed

Insights

Fourier Transform InfraRed (FTIR) spectroscopy differentiates tuberculin purified protein derivatives (PPDs) based on their source mycobacteria. This method allows for specific batch characterization and quality control of PPDs used in diagnosing mycobacterial infections.

Area of Science:

  • Veterinary Immunology
  • Microbiology
  • Analytical Chemistry

Background:

  • Tuberculins purified protein derivatives (PPDs) are crucial for diagnosing mycobacterial infections in humans and animals.
  • Bovine PPD (PPDB), Avian PPD (PPDA), and Johnin PPD (PPDJ) are derived from specific mycobacterial species.
  • Limited knowledge exists regarding the precise composition of PPDs despite their long history of use.

Purpose of the Study:

  • To evaluate Fourier Transform InfraRed (FTIR) spectroscopy for differentiating PPDB, PPDA, and PPDJ.
  • To establish FTIR as a tool for characterizing PPD batches and assessing their composition.

Main Methods:

  • Precipitation of PPDs from heat-treated mycobacteria (Mycobacterium bovis, Mycobacterium avium, M. avium subsp. paratuberculosis).
  • Analysis of PPD samples using FTIR spectroscopy.
  • Correlation of FTIR spectra with the phylogenetic characteristics of the source mycobacteria.

Main Results:

  • FTIR spectroscopy revealed distinct spectral profiles for PPDB, PPDA, and PPDJ.
  • These specific profiles correlated with the phylogenetic origins of the mycobacteria used for PPD production.
  • Nitrogen content in PPD preparations was found to be related to their IR spectra.

Conclusions:

  • FTIR spectroscopy is a suitable method for differentiating and characterizing different PPDs.
  • The technique enables efficient control of PPD production, ensuring batch consistency and quality.
  • Standardized FTIR analysis protocols can be developed for PPD quality assessment.
Keywords:
FTIRcharacterizationmycobacteriapurified protein derivativetuberculosis

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