Related Experiment Video
Updated: Jan 20, 2026
Reliability and Validity: Consistency, Reproducibility and Accuracy
Development and validation of a reliable LC-MS/MS method for quantitative analysis of usnic acid in Cladonia uncialis
Natalia Sveshnikova1, Tao Yuan2, Jamie M Warren2
1School of Science and the Environment, Grenfell Campus, Memorial University of NL, 20 University Drive, Corner Brook, NL, A2H 5G4, USA.
Objective:
The purpose of this study was to develop and validate a specific and sensitive liquid chromatography tandem mass-spectrometry method for quantification of usnic acid concentration in the lichen, Cladonia uncialis, suitable for detection of relatively small fluctuations of usnic acid concentration in response to environmental changes.
Results:
The resulting method was fully validated according to international guidelines and demonstrated good selectivity and sensitivity with minor levels of a matrix effect and high accuracy.
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