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Eyestalk Ablation to Increase Ovarian Maturation in Mud Crabs04:28

Eyestalk Ablation to Increase Ovarian Maturation in Mud Crabs

Two eyestalk ablation protocols (i.e., cauterization and surgery approaches) were performed on anesthetized female crabs. The eyestalk ablation of mud crabs hastened the ripening of ovaries without decreasing the survival...
2.6K
Fate Mapping of Human Embryonic Stem Cells by Teratoma Formation08:56

Fate Mapping of Human Embryonic Stem Cells by Teratoma Formation

Directed differentiation of hESCs into specific cells has generated much interest in regenerative medicine. We provide a concise, step-by-step protocol for determining the in vivo fate of selected hESCs that provides a valuable tool for characterizing tissue-specific reagents for cell-based...
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Teratoma Generation in the Testis Capsule05:24

Teratoma Generation in the Testis Capsule

Human pluripotent stem cells (hPSCs) have the potential to treat a myriad of different diseases. The utility of these cells lies in the fact that they can differentiate into any cell type in the body. Here we describe the teratoma assay, which is used to demonstrate the pluripotence of...
21.4K
Feeder-free Derivation of Melanocytes from Human Pluripotent Stem Cells12:21

Feeder-free Derivation of Melanocytes from Human Pluripotent Stem Cells

This work describes an in vitro differentiation protocol to produce pigmented, mature melanocytes from human pluripotent stem cells via a neural crest and melanoblast intermediate stage using a feeder-free, 25 day...
10.8K
Preparation of Mitochondria from Ovarian Cancer Tissues and Control Ovarian Tissues for Quantitative Proteomics Analysis11:51

Preparation of Mitochondria from Ovarian Cancer Tissues and Control Ovarian Tissues for Quantitative Proteomics Analysis

This article presents a protocol of differential-speed centrifugation in combination with density gradient centrifugation to separate mitochondria from human ovarian cancer tissues and control ovarian tissues for quantitative proteomics analysis, resulting in a high-quality mitochondrial sample and high-throughput and high-reproducibility quantitative proteomics analysis of a human ovarian cancer mitochondrial...
6.3K
Isolating Primary Melanocyte-like Cells from the Mouse Heart07:38

Isolating Primary Melanocyte-like Cells from the Mouse Heart

In this protocol, we identified a novel population of melanocyte-like cells (also known as cardiac melanocytes) in the hearts of mice and humans that contribute to atrial arrhythmia triggers in...
10.8K