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Generating Plasma Membrane Vesicles from Bone Marrow Stem Cells03:20

Generating Plasma Membrane Vesicles from Bone Marrow Stem Cells

This video demonstrates a method to generate plasma membrane vesicles from bone marrow stem cells by passing them rapidly through a membrane filter. These PMVs show potential for cytoplasm replacement...
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A Standardized Liquid Biopsy Preanalytical Protocol for Downstream Circulating-Free DNA Applications05:26

A Standardized Liquid Biopsy Preanalytical Protocol for Downstream Circulating-Free DNA Applications

The liquid biopsy has revolutionized our approach to oncology translational studies, with sample collection, quality, and storage being crucial steps for its successful clinical application. Here we describe a standardized and validated protocol for downstream circulating-free DNA applications that can be applied in most translational research...
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In Vitro Differentiation Model of Human Normal Memory B Cells to Long-lived Plasma Cells10:26

In Vitro Differentiation Model of Human Normal Memory B Cells to Long-lived Plasma Cells

Using multi-step culture systems, we report an in vitro B cell to plasma cell differentiation...
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Exosomal miRNA Analysis in Non-small Cell Lung Cancer (NSCLC) Patients' Plasma Through qPCR: A Feasible Liquid Biopsy Tool08:49

Exosomal miRNA Analysis in Non-small Cell Lung Cancer (NSCLC) Patients' Plasma Through qPCR: A Feasible Liquid Biopsy Tool

This protocol describes the feasibility to perform miRNA profiling in exosomes, released in plasma of NSCLC patients, through a commercial exosome isolation kit with Proteinase K and RNAse treatments, in order to avoid circulating miRNAs contamination and evaluate their biomarker features in...
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Bone Marrow Aspiration: A Method to Obtain Bone Marrow to Examine Cell Morphology03:56

Bone Marrow Aspiration: A Method to Obtain Bone Marrow to Examine Cell Morphology

This video describes the technique of aspirating bone marrow from iliac spine and smear preparation for biopsy. In the example, we perform bone marrow aspiration from an acute myeloid leukemia (AML) patient and examine the cells to check for measurable residual disease (MRD), an important prognostic...
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Basic Research in Plasma Medicine - A Throughput Approach from Liquids to Cells07:37

Basic Research in Plasma Medicine - A Throughput Approach from Liquids to Cells

A high-throughput cold physical plasma treatment protocol of liquids and cells is shown. It involves setting up different feed gas compositions for plasma ignition, measuring the plasma's emission spectra, and the subsequent analysis of liquids and cellular activity after plasma...
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