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Single Cell Measurements of Vacuolar Rupture Caused by Intracellular Pathogens10:39

Single Cell Measurements of Vacuolar Rupture Caused by Intracellular Pathogens

We describe a method for tracking the endomembrane rupture elicited by the intracellular bacteria Shigella flexneri and Mycobacterium tuberculosis upon host cell invasion. Our assay makes use of CCF4, a host cytoplasmic FRET probe in live or fixed cells. This reporter is degraded by an enzyme activity present on the bacterial...
14.0K
Double Labeling Immunofluorescence using Antibodies from the Same Species to Study Host-Pathogen Interactions07:35

Double Labeling Immunofluorescence using Antibodies from the Same Species to Study Host-Pathogen Interactions

Here, the protocol describes how to perform double labeling immunofluorescence using primary antibodies raised in the same species to study host-pathogen interactions. Also, it can include the third antibody from a different host in this protocol. This approach can be made in any cell type and...
7.6K
Use of an Optical Trap for Study of Host-Pathogen Interactions for Dynamic Live Cell Imaging09:17

Use of an Optical Trap for Study of Host-Pathogen Interactions for Dynamic Live Cell Imaging

A method is described to individually select, manipulate, and image live pathogens using an optical trap coupled to a spinning disk microscope. The optical trap provides spatial and temporal control of organisms and places them adjacent to host cells. Fluorescence microscopy captures dynamic intercellular interactions with minimal perturbation to...
13.4K
In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions10:27

In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions

The protocol submitted here explains the complete in silico pipeline needed to predict and functionally characterize circRNAs from RNA-sequencing transcriptome data studying host-pathogen...
2.0K
Use of Image Cytometry for Quantification of Pathogenic Fungi in Association with Host Cells07:58

Use of Image Cytometry for Quantification of Pathogenic Fungi in Association with Host Cells

Here, we demonstrate how image cytometry can be used for quantification of pathogenic fungi in association with host cells in culture. This technique can be used as an alternative to CFU...
13.4K
Using a Bacterial Pathogen to Probe for Cellular and Organismic-level Host Responses08:38

Using a Bacterial Pathogen to Probe for Cellular and Organismic-level Host Responses

We describe both in vitro and in vivo infection assays that can be used to analyze the activities of host-encoding...
6.3K