Related Experiment Video
Updated: Jan 20, 2026
RNA Binding Proteins and RNA Stability
Live imaging of mRNA using RNA-stabilized fluorogenic proteins
Jiahui Wu1, Sara Zaccara1, Deepak Khuperkar2
1Department of Pharmacology, Weill Cornell Medicine, Cornell University, New York, NY, USA.
Abstract:
Fluorogenic RNA aptamers bind and activate the fluorescence of otherwise nonfluorescent dyes. However, fluorogenic aptamers are limited by the small number of fluorogenic dyes suitable for use in live cells. In this communication, fluorogenic proteins whose fluorescence is activated by RNA aptamers are described. Fluorogenic proteins are highly unstable until they bind RNA aptamers inserted into messenger RNAs, resulting in fluorescent RNA-protein complexes that enable live imaging of mRNA in living cells.
Related Concept Videos
RNA Stability
mRNA Stability and Gene Expression
Cis-acting Elements involved in mRNA stability
mRNA Stability and Gene Expression
08:11Determination of In Vitro and Cellular Turn-on Kinetics for Fluorogenic RNA Aptamers
09:45Measuring Protein Stability in Living Zebrafish Embryos Using Fluorescence Decay After Photoconversion (FDAP)
11:22Measuring the Kinetics of mRNA Transcription in Single Living Cells
