Sequencing Technologies and Analyses: Where Have We Been and Where Are We Going?

A wave of technologies transformed sequencing over a decade ago into the high-throughput era, demanding research in new computational methods to analyze these data. The applications of these sequencing technologies have continuously expanded since then. The RECOMB Satellite Workshop on Massively Parallel Sequencing (RECOMB-Seq) meeting, established in 2011, brings together leading researchers in computational genomics and genomic biology to discuss emerging frontiers in algorithm development for massively parallel sequencing data. The ninth edition of this workshop was held in Washington, DC, in George Washington University on May 3 and 4, 2019. There was an exploration of several traditional topics in sequence analysis, including genome assembly, sequence alignment, and data compression, and development of methods for new sequencing technologies, including linked reads and single-molecule long-read sequencing. Here we revisit these topics and discuss the current status and perspectives of sequencing technologies and analyses.