CRISPR-Cas experiments for schools and the public

Area of Science:

• Molecular Biology
• Biotechnology
• Genetics Education

Background:

• CRISPR-Cas technology offers revolutionary gene editing capabilities.
• Broad applications span biomedicine, biotechnology, and agriculture.
• Public understanding of gene editing is crucial.

Purpose of the Study:

• To develop simple, accessible CRISPR-Cas experiments for educational purposes.
• To introduce targeted gene editing to students and non-scientists.
• To foster a positive attitude towards scientific research.

Main Methods:

• Part 1: Targeting plasmid-borne lacZ in E. coli to change colony color.
• Part 2: Analyzing CRISPR-Cas9 induced DNA breaks via colony PCR, gel electrophoresis, or restriction digest.
• Integrating practical lab work with theoretical background and tutorials.

Main Results:

• Successful demonstration of targeted gene editing in E. coli.
• Analysis confirmed CRISPR-Cas9 mediated double-strand breaks in the lacZ gene.
• The lab course effectively engaged participants aged 16-70.

Conclusions:

• The developed CRISPR-Cas experiments are robust, inexpensive, and effective for science education.
• Expert guidance is necessary for successful execution.
• Full-day lab courses positively influence participants' attitudes toward research.