Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Use of a Multi-compartment Dynamic Single Enzyme Phantom for Studies of Hyperpolarized Magnetic Resonance Agents08:59

Use of a Multi-compartment Dynamic Single Enzyme Phantom for Studies of Hyperpolarized Magnetic Resonance Agents

7.2K
A multi-compartment dynamic phantom is used to simulate some biology of interest for metabolic studies using hyperpolarized magnet resonance...
7.2K
Hyperpolarized 13C Metabolic Magnetic Resonance Spectroscopy and Imaging11:43

Hyperpolarized 13C Metabolic Magnetic Resonance Spectroscopy and Imaging

11.0K
Dynamic nuclear polarization with subsequent sample dissolution has enabled real-time studies of metabolism in biological systems. Hyperpolarized [1-13C]pyruvate was used to study lactate dehydrogenase activity in a prostate carcinoma cell line in vitro.
11.0K
Measuring the Spin-Lattice Relaxation Magnetic Field Dependence of Hyperpolarized [1-13C]pyruvate11:57

Measuring the Spin-Lattice Relaxation Magnetic Field Dependence of Hyperpolarized [1-13C]pyruvate

7.0K
We present a protocol to measure the magnetic field dependence of the spin-lattice relaxation time of 13C-enriched compounds, hyperpolarized by means of dynamic nuclear polarization, using fast field-cycled relaxometry. Specifically, we have demonstrated this with [1-13C]pyruvate, but the protocol could be extended to other hyperpolarized...
7.0K
Real-Time Metabolic Detection in Living Cells Using Hyperpolarized 13C NMR09:05

Real-Time Metabolic Detection in Living Cells Using Hyperpolarized 13C NMR

1.3K
This protocol integrates a custom-built bioreactor system with hyperpolarized ¹³C NMR spectroscopy to measure real-time pyruvate metabolism in live cells, improving reproducibility and kinetic accuracy for metabolic studies and drug screening...
1.3K
Hyperpolarized Xenon for NMR and MRI Applications16:20

Hyperpolarized Xenon for NMR and MRI Applications

20.1K
The production of hyperpolarized xenon by means of spin exchange optical pumping (SEOP) is described. This method yields a ~10000-fold enhancement of the nuclear spin polarization of Xe-129 and has applications in nuclear magnetic resonance spectroscopy and imaging. Examples of gas phase and solution state experiments are...
20.1K
Quantitative Measure of Lung Structure and Function Obtained from Hyperpolarized Xenon Spectroscopy08:23

Quantitative Measure of Lung Structure and Function Obtained from Hyperpolarized Xenon Spectroscopy

1.1K
The manuscript presents a detailed protocol for using hyperpolarized Xenon-129 chemical shift saturation recovery (CSSR) to trace pulmonary gas exchange, assess the apparent alveolar septal wall thickness, and measure the surface-to-volume ratio. The method has the potential to diagnose and monitor lung...
1.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Interaction With Surface Spins as a Contribution to Nuclear Magnetic Relaxation of Liquids Adsorbed in Mesoporous Materials.

Magnetic resonance in chemistry : MRC·2026
Same author

Evidence for the demixing of miscible binary liquids in mesoporous glass.

Physical chemistry chemical physics : PCCP·2026
Same author

Mean pore diameters of 3D printed micro-capillaries using the matrix pencil method.

Magnetic resonance letters·2026
Same author

Usability of Amorphous Manganese Oxide for Assessing the Proteoglycan Content in Articular Cartilage.

Magnetic resonance in chemistry : MRC·2025
Same author

Binary fluids in mesoporous materials: Phase separation studied by NMR relaxation and diffusion.

Magnetic resonance letters·2025
Same author

Dipolar NMR relaxation of adsorbates on surfaces of controlled wettability.

Magnetic resonance letters·2025

Related Experiment Video

Updated: Jan 20, 2026

Use of a Multi-compartment Dynamic Single Enzyme Phantom for Studies of Hyperpolarized Magnetic Resonance Agents
08:59

Use of a Multi-compartment Dynamic Single Enzyme Phantom for Studies of Hyperpolarized Magnetic Resonance Agents

Published on: April 15, 2016

7.2K

X-nuclei hyperpolarization for studying molecular dynamics by DNP-FFC.

Bulat Gizatullin1, Carlos Mattea1, Siegfried Stapf1

  • 1FG Technische Physik II/Polymerphysik, Technische Universität Ilmenau, D-98684 Ilmenau, Germany.

Journal of Magnetic Resonance (San Diego, Calif. : 1997)
|September 1, 2019
PubMed
Summary

Dynamic Nuclear Polarization (DNP) enhances NMR data quality. This study extends DNP to X-nuclei (like 2H, 7Li, 13C) for improved molecular dynamics studies, achieving significant signal boosts.

Keywords:
DNPFFCHyperpolarizationNMROverhauser effectTEMPOX-nuclei

More Related Videos

Hyperpolarized 13C Metabolic Magnetic Resonance Spectroscopy and Imaging
11:43

Hyperpolarized 13C Metabolic Magnetic Resonance Spectroscopy and Imaging

Published on: December 30, 2016

11.0K
Measuring the Spin-Lattice Relaxation Magnetic Field Dependence of Hyperpolarized [1-13C]pyruvate
11:57

Measuring the Spin-Lattice Relaxation Magnetic Field Dependence of Hyperpolarized [1-13C]pyruvate

Published on: September 13, 2019

7.0K

Related Experiment Videos

Last Updated: Jan 20, 2026

Use of a Multi-compartment Dynamic Single Enzyme Phantom for Studies of Hyperpolarized Magnetic Resonance Agents
08:59

Use of a Multi-compartment Dynamic Single Enzyme Phantom for Studies of Hyperpolarized Magnetic Resonance Agents

Published on: April 15, 2016

7.2K
Hyperpolarized 13C Metabolic Magnetic Resonance Spectroscopy and Imaging
11:43

Hyperpolarized 13C Metabolic Magnetic Resonance Spectroscopy and Imaging

Published on: December 30, 2016

11.0K
Measuring the Spin-Lattice Relaxation Magnetic Field Dependence of Hyperpolarized [1-13C]pyruvate
11:57

Measuring the Spin-Lattice Relaxation Magnetic Field Dependence of Hyperpolarized [1-13C]pyruvate

Published on: September 13, 2019

7.0K

Area of Science:

  • Nuclear Magnetic Resonance (NMR) Spectroscopy
  • Physical Chemistry
  • Biophysics

Background:

  • Dynamic Nuclear Polarization (DNP) significantly enhances Nuclear Magnetic Resonance (NMR) sensitivity and selectivity.
  • Fast Field Cycling (FFC) relaxometry combined with DNP enables molecular dynamics studies, especially when signal-to-noise ratios are critical.
  • Extending DNP beyond proton (1H) NMR to other nuclei (X-nuclei) is crucial for broader applications.

Purpose of the Study:

  • To demonstrate the extension of NMR relaxation dispersion beyond 1H NMR using DNP hyperpolarization of X-nuclei.
  • To investigate the feasibility of observing the Overhauser effect for X-nuclei (2H, 7Li, 13C) in simple liquids and saline solutions.
  • To develop a method for reconstructing original relaxation dispersion data by eliminating radical-induced relaxivity.

Main Methods:

  • Dynamic Nuclear Polarization (DNP) utilizing nitroxide radicals.
  • Fast Field Cycling (FFC) relaxometry.
  • Observation of the Nuclear Overhauser Effect (NOE) for 2H, 7Li, and 13C nuclei.
  • Advanced data processing for reconstructing relaxation dispersion curves.

Main Results:

  • Successful hyperpolarization of 2H, 7Li, and 13C nuclei was achieved via the Overhauser effect using nitroxide radicals.
  • Substantial NMR signal enhancements, up to several hundredfold, were observed for the studied X-nuclei.
  • An advanced reconstruction method effectively removed the confounding effects of radical relaxivity, allowing for the recovery of intrinsic relaxation dispersion.

Conclusions:

  • NMR relaxation dispersion studies can be effectively extended to X-nuclei using DNP and FFC relaxometry.
  • This approach significantly boosts NMR signal-to-noise ratios, making molecular dynamics studies more feasible.
  • The developed methods allow for accurate characterization of molecular dynamics even in the presence of paramagnetic agents.