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miRNA Extraction: A Method to Extract miRNA from Plasma Sample03:59

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Related Experiment Video

Updated: Jan 20, 2026

miRNA Extraction: A Method to Extract miRNA from Plasma Sample
03:59

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Developing new TB biomarkers, are miRNA the answer?

Jessica L Pedersen1, Nilesh J Bokil1, Bernadette M Saunders1

  • 1School of Life Sciences, Faculty of Science, University of Technology Sydney, 15 Broadway, Ultimo, Sydney, 2007, Australia.

Tuberculosis (Edinburgh, Scotland)
|September 1, 2019
PubMed
Summary
This summary is machine-generated.

Developing reliable non-sputum diagnostics for tuberculosis (TB) remains challenging. While microRNAs (miRNAs) show potential, current research lacks a reproducible TB biomarker signature, necessitating further validation across diverse populations.

Keywords:
Circulating biomarkersDiagnosticsTreatmentTuberculosismicroRNA

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Area of Science:

  • Biomarker discovery
  • Molecular diagnostics
  • Tuberculosis research

Background:

  • Global tuberculosis (TB) control is hampered by the absence of simple, non-sputum diagnostic methods.
  • Previous investigations into circulating host proteins and mRNA as TB biomarkers yielded insufficient diagnostic accuracy.
  • Circulating microRNAs (miRNAs) have emerged as a promising area for TB disease biomarker development.

Purpose of the Study:

  • To systematically review published studies on miRNA biomarkers for tuberculosis.
  • To determine if a reproducible miRNA signature for TB disease diagnosis can be identified from existing literature.

Main Methods:

  • Comprehensive literature search for miRNA profiling studies in TB patients versus healthy controls.
  • Analysis of differentially expressed miRNAs identified across multiple studies.
  • Validation of candidate miRNAs using quantitative polymerase chain reaction (qPCR).

Main Results:

  • 15 miRNA profiling studies identified 894 differentially expressed miRNAs between TB patients and controls.
  • qPCR validation confirmed 53 differentially expressed miRNAs.
  • Crucially, only 8 miRNAs were consistently identified in two or more studies, indicating a lack of a reproducible signature.

Conclusions:

  • Tuberculosis is associated with widespread changes in miRNA expression.
  • Environmental factors, tissue source, ethnicity, and disease extent influence miRNA expression patterns.
  • Coordinated, multi-population validation studies are essential to advance miRNA-based TB biomarker development.