Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Outer Layers of the Cell Envelope01:18

Outer Layers of the Cell Envelope

955
The outermost layers of prokaryotic cells play a critical role in their survival, virulence, and interaction with the environment. These layers, often composed of polysaccharides, polypeptides, or proteins, form protective and adhesive structures that vary in organization and function.Capsules and Slime LayersCapsules are highly organized, tightly bound layers that firmly attach to the bacterial cell wall. Capsules are usually made of polysaccharides, though some are made of polypeptides. These...
955
Conformational Evaluation of HIV-1 Trimeric Envelope Glycoproteins Using a Cell-based ELISA Assay07:10

Conformational Evaluation of HIV-1 Trimeric Envelope Glycoproteins Using a Cell-based ELISA Assay

14.7K
Understanding viral surface antigens conformations is required to evaluate antibody neutralization and guide the design of effective vaccine immunogens. Here we describe a cell-based ELISA assay that allows the study of the recognition of trimeric HIV-1 Env expressed at the surface of transfected cells by specific anti-Env...
14.7K
Averaging of Viral Envelope Glycoprotein Spikes from Electron Cryotomography Reconstructions using Jsubtomo08:29

Averaging of Viral Envelope Glycoprotein Spikes from Electron Cryotomography Reconstructions using Jsubtomo

12.6K
An approach is presented for determining structures of viral membrane glycoprotein complexes using a combination of electron cryo-tomography and sub-tomogram averaging with the computational package...
12.6K
Determination of Molecular Structures of HIV Envelope Glycoproteins using Cryo-Electron Tomography and Automated Sub-tomogram Averaging07:29

Determination of Molecular Structures of HIV Envelope Glycoproteins using Cryo-Electron Tomography and Automated Sub-tomogram Averaging

42.0K
The protocol describes a high-throughput approach to determining structures of membrane proteins using cryo-electron tomography and 3D image processing. It covers the details of specimen preparation, data collection, data processing and interpretation, and concludes with the production of a representative target for the approach, the HIV-1 Envelope glycoprotein. These computational procedures are designed in a way that enables researchers and students to work remotely and contribute to data...
42.0K
Production of High-Titer Infectious Influenza Pseudotyped Particles with Envelope Glycoproteins from Highly Pathogenic H5N1 and Avian H7N9 Viruses08:10

Production of High-Titer Infectious Influenza Pseudotyped Particles with Envelope Glycoproteins from Highly Pathogenic H5N1 and Avian H7N9 Viruses

8.8K
This protocol describes an experimental process to produce high-titer infectious viral pseudotyped particles (pp) with envelope glycoproteins from two influenza A strains and how to determine their infectivity. This protocol is highly adaptable to develop pps of any other type of enveloped viruses with different envelope...
8.8K
High-Speed Human Temporal Bone Sectioning for the Assessment of COVID-19-Associated Middle Ear Pathology03:42

High-Speed Human Temporal Bone Sectioning for the Assessment of COVID-19-Associated Middle Ear Pathology

2.6K
This article describes a technique for rapid human temporal bone sectioning that utilizes a microsaw with twin diamond blades to generate thin slices for rapid decalcification and analysis of temporal bone immunohistochemistry.
2.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Binaural sensitivity and processing of envelope-based interaural difference cues by bilateral cochlear implant users with perilingual and postlingual onset of deafness.

The Journal of the Acoustical Society of America·2026
Same author

To Ear and Hearing Reviewers: Thank You.

Ear and hearing·2026
Same author

Best cochlear locations for delivering interaural timing cues in electric hearing.

Communications medicine·2026
Same author

To Ear and Hearing Reviewers: Thank You.

Ear and hearing·2026
Same author

Microbial community dynamics over large spatial and environmental gradients in a subtropical ocean basin.

Applied and environmental microbiology·2026
Same author

The clinical effective of sodium/glucose co-transporter-2 inhibitors on admission frequency, duration and use of acute non-invasive ventilation in patients with chronic obstructive pulmonary disease: A single-centre 24-month retrospective observational study in a UK tertiary care centre.

Clinical medicine (London, England)·2025
Same journal

Segmental vs phrase-level creak in Polish: An acoustic analysis.

The Journal of the Acoustical Society of America·2026
Same journal

Interaction of near-wall bubble arrays with acoustic waves induced by an oscillating rigid wall.

The Journal of the Acoustical Society of America·2026
Same journal

Ultra-broadband underwater acoustic projector based on transverse resonance orthogonal beam (TROB) mode and acoustic matching layer technique.

The Journal of the Acoustical Society of America·2026
Same journal

Fine-scale quantitative analysis of bowhead whale (Balaena mysticetus) song shows varying stability of song types.

The Journal of the Acoustical Society of America·2026
Same journal

High-resolution depth estimation for multiple wideband sources in deep sea via sparse Bayesian learninga).

The Journal of the Acoustical Society of America·2026
Same journal

Depression markers in speech: An approach based on tract variables dynamics.

The Journal of the Acoustical Society of America·2026
See all related articles

Related Experiment Video

Updated: Jan 20, 2026

Conformational Evaluation of HIV-1 Trimeric Envelope Glycoproteins Using a Cell-based ELISA Assay
07:10

Conformational Evaluation of HIV-1 Trimeric Envelope Glycoproteins Using a Cell-based ELISA Assay

Published on: September 14, 2014

14.7K

Asymmetric temporal envelope encoding: Implications for within- and across-ear envelope comparison.

Sean R Anderson1, Alan Kan1, Ruth Y Litovsky1

  • 1Waisman Center, University of Wisconsin-Madison, Madison, Wisconsin 53705, USA.

The Journal of the Acoustical Society of America
|September 2, 2019
PubMed
Summary
This summary is machine-generated.

Reduced amplitude modulation depth impaired the ability to detect differences in sound rates, suggesting poor temporal coding hinders auditory comparisons. This impacts understanding sound in noisy environments.

More Related Videos

Outer Layers of the Cell Envelope
01:18

Outer Layers of the Cell Envelope

Published on: July 28, 2025

955
Averaging of Viral Envelope Glycoprotein Spikes from Electron Cryotomography Reconstructions using Jsubtomo
08:29

Averaging of Viral Envelope Glycoprotein Spikes from Electron Cryotomography Reconstructions using Jsubtomo

Published on: October 21, 2014

12.6K

Related Experiment Videos

Last Updated: Jan 20, 2026

Conformational Evaluation of HIV-1 Trimeric Envelope Glycoproteins Using a Cell-based ELISA Assay
07:10

Conformational Evaluation of HIV-1 Trimeric Envelope Glycoproteins Using a Cell-based ELISA Assay

Published on: September 14, 2014

14.7K
Outer Layers of the Cell Envelope
01:18

Outer Layers of the Cell Envelope

Published on: July 28, 2025

955
Averaging of Viral Envelope Glycoprotein Spikes from Electron Cryotomography Reconstructions using Jsubtomo
08:29

Averaging of Viral Envelope Glycoprotein Spikes from Electron Cryotomography Reconstructions using Jsubtomo

Published on: October 21, 2014

12.6K

Area of Science:

  • Auditory Neuroscience
  • Psychoacoustics
  • Signal Processing in Hearing

Background:

  • Effective sound source separation requires precise spectro-temporal comparisons.
  • Hearing impairment can degrade temporal encoding, limiting these comparisons.
  • Understanding temporal coding deficits is crucial for auditory perception research.

Purpose of the Study:

  • To investigate how reduced temporal coding affects the ability to discriminate amplitude modulation (AM) rates.
  • To examine if manipulating AM depth impacts spectro-temporal comparisons within and across cochlear places.
  • To explore the relationship between temporal representation quality and auditory processing.

Main Methods:

  • Sinusoidally amplitude modulated (SAM) tones were used with varying AM depths (50% to 20%) in normal-hearing listeners.
  • Listeners judged differences in AM rates for stimuli presented to different cochlear places, within or across ears.
  • Amplitude modulation depth was reduced for one tone in a pair to simulate degraded temporal encoding.

Main Results:

  • Reducing AM depth from 50% to 20% for one tone significantly decreased sensitivity to AM rate differences.
  • Auditory discrimination performance was highest for AM rates near 90 Hz.
  • Sensitivity depended on the specific cochlear places-of-stimulation being compared.

Conclusions:

  • Degraded temporal representations in the auditory nerve can impair the ability to compare temporal information across different cochlear locations.
  • These findings highlight the importance of intact temporal coding for effective sound segregation.
  • The results have implications for understanding hearing impairment and developing auditory aids.