Goniometer for determination of the spectrally resolved scattering phase function of suspended particles

An apparatus for direct determination of the spectral resolved scattering phase function of suspensions and emulsions is described. The system consists of a polychromatic xenon lamp as a light source and a spectrometer as a detector. Together with a stepper motor, the system enables spectrally and angularly resolved measurements in the range of 450 nm-950 nm and 10°-170°, respectively. A model for light propagation inside the cuvette in the regime of single scattering, which also takes the spectral dimension into account, was developed. A postprocessing algorithm applying the model and an extrapolation to the measured angular data allows a direct determination of the complete scattering phase function. By comparing measurements on polystyrene microspheres with Mie theory, the concept of the presented instrument was validated. Finally, the method was used to determine the scattering phase function of different types and brands of soybean oil emulsions such as Intralipid or Lipovenös. The measured scattering phase functions were then used to calculate the corresponding Legendre moments to an order of 20 and for wavelengths between 450 nm and 750 nm (available online), which besides the anisotropy factor also allow the determination of higher order factors such as gamma.