Development of a compact high-voltage pulser for hypervelocity microparticles injector

Munan Lin1, Jinlu Sun1, Xia Zhan2

  • 1Peking University Shenzhen Graduate School, Peking University, No. 2199, Lishui Road, Nanshan District, Shenzhen, 518055, China.

Summary

A novel high-voltage pulser was developed using a zero-voltage-switching (ZVS) circuit and a diode split flyback transformer. This system successfully generated high-voltage DC pulses for a hypervelocity microparticle injector, enabling microparticle ejection.

Related Concept Videos

Visualization of DNA Compaction in Cyanobacteria by High-voltage Cryo-electron Tomography09:47

Visualization of DNA Compaction in Cyanobacteria by High-voltage Cryo-electron Tomography

This protocol describes how to visualize the transient DNA compaction in cyanobacteria. Synchronous cultivation, monitoring by fluorescence microscopy, rapid freezing, and high voltage cryo-electron tomography are used. A protocol for these methodologies is presented, and future applications and developments are...
9.7K
Routine Screening Method for Microparticles in Platelet Transfusions09:49

Routine Screening Method for Microparticles in Platelet Transfusions

Platelet inventory management based on screening microparticle content in platelet concentrates is a new quality improvement initiative in hospital blood banks. The goal is to differentiate activated from non-activated platelets to optimize the use of platelets. Providing non-activated platelets to hematology-oncology patients might reduce their high risk to become...
16.5K
A High-performance Compact Photoacoustic Tomography System for In Vivo Small-animal Brain Imaging05:32

A High-performance Compact Photoacoustic Tomography System for In Vivo Small-animal Brain Imaging

A compact pulsed-laser diode-based photoacoustic tomography (PLD-PAT) system for high-speed in vivo brain imaging in small animals is...
11.0K
Compact Bone01:27

Compact Bone

Most bones contain compact and spongy osseous tissue, but their distribution and concentration vary based on the bone's overall function.
Compact bone, also called cortical bone, is the denser, stronger of the two types of bone tissue. It is found under the periosteum and in the diaphyses of long bones, where it provides support and protection. The microscopic structural unit of compact bone is called an osteon, or haversian system. Each osteon is composed of concentric rings of calcified...
16.1K
Porous Silicon Microparticles for Delivery of siRNA Therapeutics08:31

Porous Silicon Microparticles for Delivery of siRNA Therapeutics

Delivery remains the main challenge for the therapeutic implementation of small interfering RNA (siRNA). This protocol involves the use of a multifunctional and biocompatible siRNA delivery platform, consisting of arginine and polyethylenimine grafted porous silicon...
11.5K
Using the Gene Pulser MXcell Electroporation System to Transfect Primary Cells with High Efficiency12:55

Using the Gene Pulser MXcell Electroporation System to Transfect Primary Cells with High Efficiency

This procedure shows how to use the Gene Pulser MXcell electroporation system to rapidly and easily identify the best electroporation conditions for mouse embryonic fibroblasts (MEFs) or other primary cells. Considerations for troubleshooting are also discussed in the associated...
24.7K