Related Experiment Video
Updated: Jan 20, 2026

Measuring Glutathione-induced Feeding Response in Hydra
Published on: November 16, 2014
Structurally regular arrangement induced fluorescence enhancement and specific recognition for glutathione of a
Xueying Yu1, Kangnan Wang1, Miaomiao Xing1
1School of Materials Science and Engineering, University of Jinan, Jinan, 250022, Shandong, China.
Abstract:
Glutathione (GSH) is an important antioxygen and free radical scavenger in the organism. Level of GSH in vivo is associated with many diseases and specific recognition for GSH is very important. Here, a pyrene chalcone derivative 1 1-(2-hydroxyphenyl)-3-(1-pyrenyl)-2-propen-1-one as specific probe for GSH was developed. The probe can give rise to rapid blue fluorescence enhancement for GSH based on Michael addition reaction in pure PBS solution with high sensitivity, fast response rate and high specificity. The compound also can be applied for GSH detection in HeLa cell. Simultaneously, the compound exhibits blue fluorescence emission enhancement in methanol-water (1:1, v/v) solution with fluorescence quantum yield being 0.45 due to the competition of water molecules for hydrogen bonds between hydroxyl and carbonyl and the formation of structurally regular rodlike crystals, which allows regulating fluorescence emission by different solvent condition.
More Related Videos
Related Concept Videos
08:10Measuring Glutathione-induced Feeding Response in Hydra
11:19Inducing a Site Specific Replication Blockage in E. coli Using a Fluorescent Repressor Operator System
12:58Fluorescence Recovery after Photobleaching of Yellow Fluorescent Protein Tagged p62 in Aggresome-like Induced Structures
06:59Subtype-specific Optical Action Potential Recordings in Human Induced Pluripotent Stem Cell-derived Ventricular Cardiomyocytes
07:56Utilizing Time-Resolved Protein-Induced Fluorescence Enhancement to Identify Stable Local Conformations One α-Synuclein Monomer at a Time
09:04Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids

