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Microsampling with cotton thread: Storage and ultra-sensitive analysis by thread spray mass Spectrometry.

Devin J Swiner1, Sierra Jackson1, George R Durisek1

  • 1Department of Chemistry and Biochemistry, The Ohio State University, Columbus, OH, 43110, USA.

Analytica Chimica Acta
|September 2, 2019
PubMed
Summary

This study introduces a novel thread-based method for stabilizing and analyzing small blood volumes at room temperature. This approach achieves ultra-sensitive detection of analytes, with applications in forensics and clinical studies.

Keywords:
Analytical methodsDried blood storageMass spectrometryMicrosamplingThread spray

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Area of Science:

  • Analytical Chemistry
  • Biomaterials Science

Background:

  • Analyzing small biofluid volumes with low analyte concentrations is difficult.
  • Complex matrices in biofluids pose analytical challenges.

Purpose of the Study:

  • To develop an integrated thread-based platform for stabilizing and analyzing small blood volumes.
  • To enable direct, room-temperature, dry-state storage and analysis of biofluids.

Main Methods:

  • Developed an integrated thread-based microsampling platform.
  • Utilized thread spray mass spectrometry for direct analysis.
  • Demonstrated in-situ in-capillary blood processing from hydrophobic threads.

Main Results:

  • Successfully stabilized small blood volumes in a dry-state at room temperature for 42 days.
  • Achieved ultra-sensitive detection limits (parts-per-quadrillion) for diazepam and cocaine.
  • Validated sensitivity by analyzing minimal tissue-like residues.

Conclusions:

  • The thread-based platform offers a novel solution for biofluid microsampling, storage, and analysis.
  • This technology has significant potential for applications in forensic science and clinical diagnostics.
  • The method enables highly sensitive detection of analytes in complex biological matrices.