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Source: Wei, D., et al., Production of Chemicals by Klebsiella pneumoniae Using Bamboo Hydrolysate as Feedstock. J. Vis. Exp. (2017)This video demonstrates the microbial conversion of bamboo-derived sugars into 2,3-butanediol by culturing Klebsiella pneumoniae in a hydrolysate-based medium under microaerobic and mildly acidic...
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Related Experiment Video

Updated: Jan 20, 2026

Microaerobic Fermentation of Bamboo Hydrolysate by Klebsiella pneumoniae
03:14

Microaerobic Fermentation of Bamboo Hydrolysate by Klebsiella pneumoniae

Published on: November 28, 2025

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Complement mediated Klebsiella pneumoniae capsule changes.

Trine S Jensen1, Katharina V Opstrup1, Gunna Christiansen2

  • 1Department of Health Science and Technology, Aalborg University, Fredrik Bajers Vej 3b, 9220, Aalborg, Denmark.

Microbes and Infection
|September 2, 2019
PubMed
Summary
This summary is machine-generated.

Multidrug-resistant Klebsiella pneumoniae infections are a growing threat. This study reveals how the bacterium

Keywords:
Bacterial capsulesComplement systemKlebsiella pneumoniaeMembrane attack complexSerum resistance

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Last Updated: Jan 20, 2026

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Separating Bacteria by Capsule Amount Using a Discontinuous Density Gradient
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Area of Science:

  • Microbiology
  • Immunology
  • Infectious Diseases

Background:

  • Klebsiella pneumoniae is an opportunistic pathogen causing severe infections like sepsis.
  • Multidrug resistance in K. pneumoniae complicates treatment and increases mortality.
  • Understanding host-pathogen interactions is crucial for developing new therapies.

Purpose of the Study:

  • To investigate the interaction between clinical K. pneumoniae isolates and human serum.
  • To elucidate mechanisms of immune evasion by K. pneumoniae.
  • To determine how complement activation affects K. pneumoniae capsule structure.

Main Methods:

  • Characterization of ten clinical K. pneumoniae isolates from bacteremia patients.
  • Investigation of isolate interactions with human serum.
  • Analysis of complement activation pathways and deposition.
  • Morphological analysis of bacterial capsules and outer membranes.

Main Results:

  • All K. pneumoniae isolates activated the alternative complement pathway.
  • Seven isolates exhibited serum resistance, with complement deposition in their capsules.
  • Capsule changes and shedding were observed in serum-resistant isolates, preventing lysis.
  • One serum-sensitive isolate showed complement activation across multiple pathways, leading to lysis and membrane attack complex formation.

Conclusions:

  • The capsule of serum-resistant K. pneumoniae acts as a defense mechanism against complement-mediated lysis.
  • K. pneumoniae employs capsule modifications to evade host immune responses.
  • Targeting capsule-complement interactions may offer novel therapeutic strategies against K. pneumoniae infections.