Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Salmonella typhimurium LT2 metF operator mutations.

G V Stauffer1, L T Stauffer

  • 1Department of Microbiology, University of Iowa, Iowa City 52242.

Molecular & General Genetics : MGG
|September 1, 1988
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Role of the RNA polymerase alpha subunits in MetR-dependent activation of metE and metH: important residues in the C-terminal domain and orientation requirements within RNA polymerase.

Journal of bacteriology·2000
Same author

The gcvB gene encodes a small untranslated RNA involved in expression of the dipeptide and oligopeptide transport systems in Escherichia coli.

Molecular microbiology·2000
Same author

GcvA-mediated activation of gcvT-lacZ expression involves the carboxy-terminal domain of the alpha subunit of RNA polymerase.

FEMS microbiology letters·1999
Same author

The cyclic AMP receptor protein is dependent on GcvA for regulation of the gcv operon.

Journal of bacteriology·1999
Same author

Mutational analysis of the transcriptional regulator GcvA: amino acids important for activation, repression, and DNA binding.

Journal of bacteriology·1998
Same author

Promoter characterization and constitutive expression of the Escherichia coli gcvR gene.

Journal of bacteriology·1998

Researchers identified cis-acting mutations affecting Salmonella typhimurium metF gene regulation. These mutations disrupt methionine control via the metJ gene product but not vitamin B12 control via the metH gene product.

Area of Science:

  • Microbiology
  • Molecular Biology
  • Genetics

Background:

  • The Salmonella typhimurium metF gene is crucial for methionine biosynthesis.
  • Gene regulation studies are essential for understanding metabolic pathways and their control mechanisms.

Purpose of the Study:

  • To isolate and characterize cis-acting mutations affecting the regulation of the Salmonella typhimurium metF gene.
  • To elucidate the roles of metJ and metH gene products in metF gene regulation.

Main Methods:

  • Utilized an Escherichia coli lac deletion strain lysogenized with lambda phage carrying a metF-lacZ gene fusion (lambda Flac).
  • Isolated cis-acting mutations affecting metF gene expression.
  • Measured beta-galactosidase levels to assess gene regulation in wild-type and mutant strains.

Related Experiment Videos

Main Results:

  • Mutations were localized to the metF operator region.
  • The identified mutations disrupted the methionine control system mediated by the metJ gene product.
  • Vitamin B12 control via the metH gene product was not affected by these mutations.

Conclusions:

  • The metJ gene product is essential for regulating metF gene expression in response to methionine levels.
  • Negative control of the metF gene by the metH gene product and vitamin B12 is dependent on a functional metJ gene product.