Related Experiment Video
Updated: Jan 20, 2026

Author Spotlight: Cryopreservation of Whole Blood Samples for High Throughput Analysis
Published on: February 23, 2024
In Vitro Cytogenetic Assays: Chromosomal Aberrations and Micronucleus Tests
Pasquale Mosesso1, Serena Cinelli2
1Dipartimento di Scienze Ecologiche e Biologiche, Università degli Studi della Tuscia, Largo dell'Università, Viterbo, Italy. mosesso@unitus.it.
Abstract:
Chromosome damage is a very important indicator of genetic damage relevant to environmental and clinical studies. Detailed descriptions of the protocols used for detection of chromosomal aberrations induced by genotoxic agents in vitro both in the presence or absence of rat liver-derived metabolizing systems are given in this chapter. Structural chromosomal aberrations that can be observed and quantified at metaphases are described here. For the detection of chromosomal damage (fragments or whole chromosome) in interphase, the micronucleus test can be used, and a description of this test is also presented. Criteria for determining a positive result using appropriate statistical methods are described.
Related Concept Videos
06:14The Micronucleus Assay on Cryopreserved Whole Blood
08:48Analysis of the Ambient Particulate Matter-induced Chromosomal Aberrations Using an In Vitro System
Cytogenetics
12:56An Automated Method to Perform The In Vitro Micronucleus Assay using Multispectral Imaging Flow Cytometry
11:08Exploring X Chromosomal Aberrations in Ovarian Cells by Using Fluorescence In Situ Hybridization
08:25Advanced 3D Liver Models for In vitro Genotoxicity Testing Following Long-Term Nanomaterial Exposure
