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Related Concept Videos

Assaying DNA Damage in Hippocampal Neurons Using the Comet Assay10:00

Assaying DNA Damage in Hippocampal Neurons Using the Comet Assay

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The comet assay is an efficient way of detecting single- and double-strand breaks, including alkali-labile sites and DNA-DNA/DNA-protein cross-links on the DNA in all cells including hippocampal neurons. The method takes advantage of the differential migration of DNA in an electric field due to differences in amount of DNA...
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Evaluating In Vitro DNA Damage Using Comet Assay10:43

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The comet assay is an efficient method to detect DNA damage including single and double-stranded DNA breaks. We describe alkaline and neutral comet assays to measure DNA damage in cancer cells to evaluate the therapeutic effect of...
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Use of Frozen Tissue in the Comet Assay for the Evaluation of DNA Damage11:30

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This protocol describes several procedures for preparing high quality frozen tissue samples at the time of necropsy for use in the comet assay to assess DNA damage: 1) minced tissue, 2) scraped epithelial cells from the gastrointestinal tract, and 3) cubed tissue samples, requiring homogenization using a tissue mincing...
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In Vivo Alkaline Comet Assay and Enzyme-modified Alkaline Comet Assay for Measuring DNA Strand Breaks and Oxidative DNA Damage in Rat Liver10:38

In Vivo Alkaline Comet Assay and Enzyme-modified Alkaline Comet Assay for Measuring DNA Strand Breaks and Oxidative DNA Damage in Rat Liver

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The alkaline comet assay measures DNA strand breaks in eukaryotic cells. By adding an Endonuclease III or human 8-oxoguanine-DNA-N-glycosylase digestion step, the assay can efficiently detect oxidative DNA damage. We describe methods for using these assays to detect DNA damage in rat...
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Comet Assay for DNA Damage Detection in Cells06:19

Comet Assay for DNA Damage Detection in Cells

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The video demonstrates the comet assay for detecting DNA damage following exposure to harmful agents. Under alkaline conditions, the DNA unwinds and denatures, and upon electrophoresis, the undamaged and damaged DNA migrate differently in the agarose gel, forming a comet-shaped structure. The slowly migrating undamaged DNA forms the circular comet head, while the rapidly migrating damaged DNA fragments form the elongated comet...
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Comet Assay as an Indirect Measure of Systemic Oxidative Stress08:23

Comet Assay as an Indirect Measure of Systemic Oxidative Stress

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Comet assay measures DNA breaks, induced by different factors. If all factors (except oxidative stress) causing DNA damage are kept constant, the amount of DNA damage is a good indirect parameter of oxidative stress. The goal of this protocol is to use comet assay for indirect measurement of oxidative...
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Related Experiment Video

Updated: Jan 20, 2026

Assaying DNA Damage in Hippocampal Neurons Using the Comet Assay
10:00

Assaying DNA Damage in Hippocampal Neurons Using the Comet Assay

Published on: December 19, 2012

28.1K

The Comet Assay in Marine Animals.

Patrizia Guidi1, Brett P Lyons2, Giada Frenzilli3

  • 1Department of Clinical and Experimental Medicine, University of Pisa, Pisa, Italy.

Methods in Molecular Biology (Clifton, N.J.)
|September 2, 2019
PubMed
Summary

The Comet assay is a versatile tool for detecting DNA damage in marine animals. This study details protocols for alkaline, mild alkaline, and neutral Comet assays to assess genetic damage in aquatic organisms.

Keywords:
Comet assayDNA strand breaksGillsHemocytesMarine animalsSingle-cell gel electrophoresis

More Related Videos

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Use of Frozen Tissue in the Comet Assay for the Evaluation of DNA Damage
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Related Experiment Videos

Last Updated: Jan 20, 2026

Assaying DNA Damage in Hippocampal Neurons Using the Comet Assay
10:00

Assaying DNA Damage in Hippocampal Neurons Using the Comet Assay

Published on: December 19, 2012

28.1K
Evaluating In Vitro DNA Damage Using Comet Assay
10:43

Evaluating In Vitro DNA Damage Using Comet Assay

Published on: October 11, 2017

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Use of Frozen Tissue in the Comet Assay for the Evaluation of DNA Damage
11:30

Use of Frozen Tissue in the Comet Assay for the Evaluation of DNA Damage

Published on: March 24, 2020

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Area of Science:

  • Marine Biology
  • Genotoxicology
  • Molecular Biology

Background:

  • The Comet assay is a widely used method for evaluating DNA damage at the cellular level.
  • Assessing DNA integrity is crucial for understanding the health and environmental impact on marine populations.
  • Existing Comet assay protocols may require adaptation for marine species due to physiological differences.

Purpose of the Study:

  • To provide optimized protocols for the Comet assay in marine animals.
  • To adapt alkaline, mild alkaline, and neutral Comet assay versions for aquatic organisms.
  • To facilitate the assessment of DNA damage in marine environmental studies.

Main Methods:

  • Detailed protocols for single-cell gel electrophoresis (Comet assay) are presented.
  • Three pH variations (alkaline pH > 13, mild alkaline pH = 12.1, neutral pH = 8) were adapted.
  • The application of these methods to marine animal cells is described.

Main Results:

  • The study successfully adapted and validated Comet assay protocols for marine organisms.
  • The described methods allow for the detection of various DNA lesions including single- and double-strand breaks and alkali-labile sites.
  • The versatility of the Comet assay across different pH conditions is demonstrated for marine samples.

Conclusions:

  • The adapted Comet assay protocols are effective for assessing DNA damage in marine animals.
  • These methods provide a valuable tool for ecotoxicological assessments and monitoring marine ecosystem health.
  • The study enhances the utility of the Comet assay in marine research by offering specific, optimized procedures.