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An Intracerebroventricular Injection to Analyze Murine Cytomegalovirus Distribution in a Neonatal Mouse Brain02:59

An Intracerebroventricular Injection to Analyze Murine Cytomegalovirus Distribution in a Neonatal Mouse Brain

This video demonstrates the intracerebroventricular injection process for studying murine cytomegalovirus (MCMV) distribution in the neonatal mouse brain. MCMV, engineered to express an enhanced green fluorescent protein (EGFP) for visibility, is injected into the lateral ventricle of a mouse pup's brain. The injected virus circulates through the cerebrospinal fluid, infecting cells in the periventricular region, including the marginal zone, choroid plexus, and subventricular...
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Histological Quantification to Determine Lung Fungal Burden in Experimental Aspergillosis09:52

Histological Quantification to Determine Lung Fungal Burden in Experimental Aspergillosis

Here we describe a protocol to determine pulmonary fungal burden in mice with invasive aspergillosis by quantification of Gomori's modified methanamine silver staining in histological sections. Use of this method resulted in comparable results with less animals compared to assessment of fungal burden by quantitative PCR of lung fungal...
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Dermoscopy Aids in the Diagnosis of Discoid Lupus Erythematosus05:39

Dermoscopy Aids in the Diagnosis of Discoid Lupus Erythematosus

Herein, we present a protocol to demonstrate the utility of dermoscopy in rheumatic diseases. In addition, we described the dermoscopic manifestations of discoid lupus erythematosus skin...
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An Immunofluorescence Method to Measure the Neutralization of Human Cytomegalovirus04:05

An Immunofluorescence Method to Measure the Neutralization of Human Cytomegalovirus

Source:Matthew J Murray1, Gary R McLean2,3, Matthew B Reeves11Institute of Immunity and Transplantation, University College London.2Cellular and Molecular Immunology Research Centre, London Metropolitan University.3National Heart and Lung Institute, Imperial College London.This video demonstrates an immunofluorescence assay designed to measure the neutralization of Human Cytomegalovirus (HCMV). Virus-specific antibodies are mixed with the virus and incubated with human fibroblast cells. The...
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Retroperitoneal Laparoscopic Debridement and Drainage for Pancreatic Abscess03:42

Retroperitoneal Laparoscopic Debridement and Drainage for Pancreatic Abscess

To improve the drainage of patients with necrotizing pancreatitis complicated with peripancreatic abscess and reduce the mortality of patients with necrotizing pancreatitis, we adopted a retroperitoneal approach for peripancreatic abscess debridement and drainage by...
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Use of In vivo Imaging to Monitor the Progression of Experimental Mouse Cytomegalovirus Infection in Neonates05:53

Use of In vivo Imaging to Monitor the Progression of Experimental Mouse Cytomegalovirus Infection in Neonates

Human Cytomegalovirus (HCMV) infection of neonates represents an important cause of mental retardation, yet the molecular events leading to virus-induced pathogenesis are still poorly understood. To investigate the dynamics of brain infection, we adapted whole-animal in vivo imaging to perform time-course analysis of neonates infected with a luciferase-recombinant...
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