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Two-dimensional NMR analyses of dynamic structures of tRNA and the regulation of codon recognition by
G Kawai1, S Yokoyama, T Hasegawa
1Department of Biophysics and Biochemistry, Faculty of Science, University of Tokyo, Japan.
Nucleic Acids Symposium Series
|January 1, 1988
Abstract:
By two-dimensional nuclear magnetic resonance (2D-NMR) spectroscopy, we analyzed dynamic structures of various tRNA species from Escherichia coli and Bacillus subtilis. Proton resonances due to the anticodon of the tRNA molecules were unambiguously identified by NOESY and 2D-HOHAHA techniques. Thus, it was found that rigidity/flexibility of the two types of modified uridines in the first position of the anticodon were certainly related with the codon recognition properties of the tRNA species.