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Immunochemical approach for the characterization of DNA damages induced by chemical carcinogens

M Tada1, T Morita

  • 1Laboratory of Biochemistry, Aichi Cancer Center, Research Institute, Osaka, Japan.

Nucleic Acids Symposium Series
|January 1, 1988
PubMed

Insights

A new mouse monoclonal antibody specifically recognizes DNA conformational changes induced by the chemical carcinogen 4-nitroquinoline 1-oxide (4NQO). This antibody offers a tool for detecting DNA damage caused by carcinogens.

Area of Science:

  • Immunology
  • Molecular Biology
  • Toxicology

Background:

  • Chemical carcinogens can induce specific conformational changes in DNA.
  • Developing tools to detect these changes is crucial for understanding carcinogenesis.

Purpose of the Study:

  • To generate and characterize a monoclonal antibody that recognizes 4-nitroquinoline 1-oxide (4NQO) modified poly(dG-dC).poly(dG-dC).
  • To assess the antibody's specificity for detecting carcinogen-induced DNA alterations.

Main Methods:

  • Immunization of mice with 4NQO-modified poly(dG-dC).poly(dG-dC).
  • Characterization using enzyme-linked immunosorbent assay (ELISA) and radioimmunoassay.
  • Testing specificity against various modified and unmodified polynucleotides.

Main Results:

  • The antibody specifically bound to 4NQO-modified poly(dG-dC).poly(dG-dC).
  • It showed no cross-reactivity with unmodified poly(dG-dC).poly(dG-dC) or 4NQO-modified DNA.
  • Slight cross-reactivity was observed with other modified polynucleotides adopting Z-conformation.

Conclusions:

  • The antibody likely recognizes a unique conformational change in poly(dG-dC).poly(dG-dC) induced by 4NQO.
  • This antibody is a valuable tool for detecting DNA conformational changes caused by chemical carcinogens.

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