Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Derivation of a Human Brain Organoid with Microglia Development10:34

Derivation of a Human Brain Organoid with Microglia Development

1.9K
We present a protocol to generate a human brain organoid with resident microglia by incorporating Induced pluripotent stem cell (iPSC)-derived hematopoietic progenitor cells (HPCs) into organoid...
1.9K
Electroporation of Sliced Human Cortical Organoids for Studies of Gene Function07:13

Electroporation of Sliced Human Cortical Organoids for Studies of Gene Function

1.9K
Here, we present the acute genetic manipulation of sliced human cortical organoids by electroporation. These cortical organoid models are particularly amenable to injection as ventricle-like structures can be readily identified after slicing, enabling the functional investigation of human cortical development, neurodevelopmental disorders, and cortical evolution.
1.9K
Robust and Highly Reproducible Generation of Cortical Brain Organoids for Modelling Brain Neuronal Senescence In Vitro05:40

Robust and Highly Reproducible Generation of Cortical Brain Organoids for Modelling Brain Neuronal Senescence In Vitro

4.5K
In this study, we provide a detailed technique for a simple yet robust cortical organoid culture system using standard feeder-free hPSC cultures. This is a rapid, efficient, and reproducible protocol for generating organoids that model aspects of brain senescence in...
4.5K
Generation of iPSC-derived Human Brain Organoids to Model Early Neurodevelopmental Disorders07:40

Generation of iPSC-derived Human Brain Organoids to Model Early Neurodevelopmental Disorders

21.4K
Modeling human brain development has been hindered due to the unprecedented complexity of neural epithelial tissue. Here, a method for the robust generation of brain organoids to delineate early events of human brain development and to model microcephaly in vitro is described.
21.4K
Modelling Zika Virus Infection of the Developing Human Brain In Vitro Using Stem Cell Derived Cerebral Organoids09:18

Modelling Zika Virus Infection of the Developing Human Brain In Vitro Using Stem Cell Derived Cerebral Organoids

11.0K
This protocol describes a technique used to model Zika virus infection of the developing human brain. Using wildtype or engineered stem cell lines, researchers may use this technique to uncover the various mechanisms or treatments that may affect early brain infection and resulting microcephaly in Zika virus-infected...
11.0K
Generation of Human Brain Organoids for Mitochondrial Disease Modeling08:09

Generation of Human Brain Organoids for Mitochondrial Disease Modeling

6.7K
We describe a detailed protocol for the generation of human induced pluripotent stem cell-derived brain organoids and their use in modeling mitochondrial...
6.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Notch Signaling Reprograms Glial Lipid Metabolism to Promote Hypoxia Resistance.

bioRxiv : the preprint server for biology·2026
Same author

Transcriptional perturbation of LINE-1 elements reveals their cis-regulatory potential.

EMBO reports·2026
Same author

Revisiting post-stimulus theta activity: evidence for an aperiodic rather than oscillatory origin.

bioRxiv : the preprint server for biology·2026
Same author

<i>Neurophotonics</i> book club: "The Secret of Secrets" by Dan Brown - a thought-provoking twist on a famous neuroscience controversy.

Neurophotonics·2026
Same author

Associations between fentanyl exposure, gut microbiome and neurodevelopmental outcomes in preterm infants.

Pediatric research·2026
Same author

Potential mechanisms and functional significance of aperiodic neural activity.

Nature human behaviour·2026

Related Experiment Video

Updated: Jan 20, 2026

Derivation of a Human Brain Organoid with Microglia Development
10:34

Derivation of a Human Brain Organoid with Microglia Development

Published on: January 17, 2025

1.9K

Complex Oscillatory Waves Emerging from Cortical Organoids Model Early Human Brain Network Development.

Cleber A Trujillo1, Richard Gao2, Priscilla D Negraes1

  • 1Department of Pediatrics/Rady Children's Hospital San Diego, School of Medicine, University of California, San Diego, La Jolla, CA 92093, USA; Department of Cellular & Molecular Medicine, School of Medicine, University of California, San Diego, La Jolla, CA 92093, USA.

Cell Stem Cell
|September 3, 2019
PubMed
Summary
This summary is machine-generated.

Human brain organoids reveal that functional network activity during development may follow genetic programming. This research models early brain maturation and electrical activity patterns.

Keywords:
cortical organoidsnetwork oscillationsphase-amplitude couplingpreterm electroencephalographysingle-cell transcriptomicsstem cells

More Related Videos

Electroporation of Sliced Human Cortical Organoids for Studies of Gene Function
07:13

Electroporation of Sliced Human Cortical Organoids for Studies of Gene Function

Published on: November 29, 2024

1.9K
Robust and Highly Reproducible Generation of Cortical Brain Organoids for Modelling Brain Neuronal Senescence In Vitro
05:40

Robust and Highly Reproducible Generation of Cortical Brain Organoids for Modelling Brain Neuronal Senescence In Vitro

Published on: May 5, 2022

4.5K

Related Experiment Videos

Last Updated: Jan 20, 2026

Derivation of a Human Brain Organoid with Microglia Development
10:34

Derivation of a Human Brain Organoid with Microglia Development

Published on: January 17, 2025

1.9K
Electroporation of Sliced Human Cortical Organoids for Studies of Gene Function
07:13

Electroporation of Sliced Human Cortical Organoids for Studies of Gene Function

Published on: November 29, 2024

1.9K
Robust and Highly Reproducible Generation of Cortical Brain Organoids for Modelling Brain Neuronal Senescence In Vitro
05:40

Robust and Highly Reproducible Generation of Cortical Brain Organoids for Modelling Brain Neuronal Senescence In Vitro

Published on: May 5, 2022

4.5K

Area of Science:

  • Neuroscience
  • Developmental Biology
  • Genetics

Background:

  • Early brain maturation involves genetically programmed structural and transcriptional changes.
  • Understanding functional network activity during initial human brain development is limited by experimental challenges.

Purpose of the Study:

  • To investigate whether functional network activity in the developing human brain follows genetically determined programs.
  • To model early human neocortex development and electrical activity using organoids.

Main Methods:

  • Development of human cortical organoids that mature over several months.
  • Monitoring of dynamic cellular changes and electrical activity, including network formation and oscillatory events.
  • Analysis of the role of glutamatergic and GABAergic signaling in network activity.

Main Results:

  • Organoids exhibited increasing electrical activity and spontaneous network formation over months.
  • Network activity showed periodic oscillations dependent on key neurotransmitters.
  • Activity patterns evolved towards spatiotemporal irregularity, mirroring preterm human electroencephalography findings.

Conclusions:

  • The development of structured network activity in a human neocortex model appears to be guided by stable genetic programming.
  • Human cortical organoids offer a platform for studying and manipulating network activity in the developing human brain.