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The Pauli Exclusion Principle03:06

The Pauli Exclusion Principle

The arrangement of electrons in the orbitals of an atom is called its electron configuration. We describe an electron configuration with a symbol that contains three pieces of information:
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Mutual Exclusivity: How Children Learn the Meanings of Words05:18

Mutual Exclusivity: How Children Learn the Meanings of Words

Source: Laboratories of Nicholaus Noles and Judith Danovitch—University of Louisville
Humans are different from other animals in many ways, but perhaps the most important differentiating factor is their ability to use language. Other animals can communicate and even understand and use language in limited ways, but trying to teach human language to a chimp or a dog takes a great deal of time and effort. In contrast, young humans acquire their native language easily, and they learn...
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Size-Exclusion Chromatography01:08

Size-Exclusion Chromatography

In size-exclusion chromatography (SEC), also known as molecular-exclusion or gel-permeation chromatography, molecules are separated based on their sizes. This technique is important for separating large molecules such as polymers and biomolecules. The two classes of micron-sized stationary phases encountered in SEC are silica particles and cross-linked polymer resin beads. Both materials are porous, but their pore sizes vary significantly.
Silica particles offer advantages such as rigidity,...
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Choosing Between Projects: Mutually Exclusive01:29

Choosing Between Projects: Mutually Exclusive

In capital budgeting, selecting between mutually exclusive projects means choosing one option from a set of options, as both cannot be pursued simultaneously. This decision significantly impacts the company's future growth and financial health.
For example, an automobile company deciding between Project A, which generates $20,000 annually for seven years, and Project B, which generates $30,000 annually for five years, may use the Net Present Value (NPV) method. After discounting future cash...
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Online Size-exclusion and Ion-exchange Chromatography on a SAXS Beamline11:09

Online Size-exclusion and Ion-exchange Chromatography on a SAXS Beamline

The determination of the solution structure of a protein by small angle X-ray scattering (SAXS) requires monodisperse samples. Here, we present two possibilities to ensure minimal delays between sample preparation and data acquisition: online size-exclusion chromatography (SEC) and online ion-exchange chromatography...
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Size Exclusion Chromatography to Analyze Bacterial Outer Membrane Vesicle Heterogeneity07:26

Size Exclusion Chromatography to Analyze Bacterial Outer Membrane Vesicle Heterogeneity

Bacterial vesicles play important roles in pathogenesis and have promising biotechnological applications. The heterogeneity of vesicles complicates analysis and use; therefore, a simple, reproducible method to separate varying sizes of vesicles is necessary. Here, we demonstrate the use of size exclusion chromatography to separate heterogeneous vesicles produced by Aggregatibacter...
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