Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Preparation of Oligomeric β-amyloid1-42 and Induction of Synaptic Plasticity Impairment on Hippocampal Slices04:41

Preparation of Oligomeric β-amyloid1-42 and Induction of Synaptic Plasticity Impairment on Hippocampal Slices

23.9K
One feature of Alzheimer's Disease is the elevation of Aβ1-42 peptide. Here we provide a protocol for preparing synthetic Aβ1-42 oligomers, which impairs hippocampal Long-Term Potentiation, a cellular correlate of memory. This procedure is useful for investigating mechanisms of Aβ-induced pathology and drug...
23.9K
Recording Synaptic Plasticity in Acute Hippocampal Slices Maintained in a Small-volume Recycling-, Perfusion-, and Submersion-type Chamber System09:51

Recording Synaptic Plasticity in Acute Hippocampal Slices Maintained in a Small-volume Recycling-, Perfusion-, and Submersion-type Chamber System

12.1K
This protocol describes the stabilization of the oxygen level in a small volume of recycled buffer and methodological aspects of recording activity-dependent synaptic plasticity in submerged acute hippocampal...
12.1K
A Rapid and Specific Microplate Assay for the Determination of Intra- and Extracellular Ascorbate in Cultured Cells11:56

A Rapid and Specific Microplate Assay for the Determination of Intra- and Extracellular Ascorbate in Cultured Cells

13.9K
Ascorbate plays numerous important roles in cellular metabolism, many of which have only come to light in recent years. Here we describe a medium-throughput, specific and inexpensive microplate assay for the determination of both intra- and extracellular ascorbate in cell culture.
13.9K
Plastic Behavior01:21

Plastic Behavior

529
A material's elastic behavior is characterized by the disappearance of stress once the load is removed, allowing the material to return to its original state. However, when stress surpasses the yield point, yielding commences, marking the onset of plastic deformation or permanent set. This change from elastic to plastic behavior is influenced by the peak stress value and the duration before the load is removed. An intriguing observation occurs when a specimen is loaded, unloaded, and...
529
Investigating Long-term Synaptic Plasticity in Interlamellar Hippocampus CA1 by Electrophysiological Field Recording14:27

Investigating Long-term Synaptic Plasticity in Interlamellar Hippocampus CA1 by Electrophysiological Field Recording

13.4K
We used recording and stimulation electrodes in longitudinal hippocampal brain slices and longitudinally positioned recording and stimulation electrodes in the dorsal hippocampus in vivo to evoke extracellular postsynaptic potentials and demonstrate long-term synaptic plasticity along the longitudinal interlamellar...
13.4K
3D Modeling of Dendritic Spines with Synaptic Plasticity07:13

3D Modeling of Dendritic Spines with Synaptic Plasticity

7.4K
The protocol develops a three-dimensional (3D) model of a dendritic segment with dendritic spines for modeling synaptic plasticity. The constructed mesh can be used for computational modeling of AMPA receptor trafficking in the long-term synaptic plasticity using the software program Blender with CellBlender and...
7.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Temporal and spatial control of phosphatidylinositides using optogenetics ameliorates behavioral deficits in an Alzheimer's disease mouse model.

Communications biology·2026
Same author

Spatial lipidomic and neuron-specific transcriptomic signatures in the nucleus accumbens reveal phospholipid dyshomeostasis in depression-related maladaptations.

Translational psychiatry·2026
Same author

Adenosine surges: A step forward in understanding antidepressant actions of ketamine.

Molecular psychiatry·2026
Same author

Vitamin D in major depressive disorder: Current evidence, possible molecular mechanisms, and future prospects.

The Journal of steroid biochemistry and molecular biology·2026
Same author

Longitudinal Changes in White Matter Hypointensities in Recurrent Late-Life Depression.

The American journal of geriatric psychiatry : official journal of the American Association for Geriatric Psychiatry·2026
Same author

Sex Differences on Social and Anxiety-Related Responses in Low-Density Lipoprotein Receptor Knockout Mice.

Molecular neurobiology·2026

Related Experiment Video

Updated: Jan 20, 2026

Preparation of Oligomeric β-amyloid1-42 and Induction of Synaptic Plasticity Impairment on Hippocampal Slices
04:41

Preparation of Oligomeric β-amyloid1-42 and Induction of Synaptic Plasticity Impairment on Hippocampal Slices

Published on: July 14, 2010

23.9K

Ascorbic acid presents rapid behavioral and hippocampal synaptic plasticity effects.

Daiane B Fraga1, Ana Paula Costa1, Gislaine Olescowicz1

  • 1Department of Biochemistry, Center of Biological Sciences, Federal University of Santa Catarina, Florianópolis, SC, Brazil.

Progress in Neuro-Psychopharmacology & Biological Psychiatry
|September 3, 2019
PubMed
Summary

Ascorbic acid (vitamin C) and ketamine, but not fluoxetine, showed rapid antidepressant effects in mice. Both treatments increased synaptic proteins and dendritic spine density in the hippocampus, suggesting shared mechanisms for rapid mood improvement.

Keywords:
Ascorbic acidDendritic spinesHippocampusKetamineSynaptic proteins

More Related Videos

Recording Synaptic Plasticity in Acute Hippocampal Slices Maintained in a Small-volume Recycling-, Perfusion-, and Submersion-type Chamber System
09:51

Recording Synaptic Plasticity in Acute Hippocampal Slices Maintained in a Small-volume Recycling-, Perfusion-, and Submersion-type Chamber System

Published on: January 1, 2018

12.1K
A Rapid and Specific Microplate Assay for the Determination of Intra- and Extracellular Ascorbate in Cultured Cells
11:56

A Rapid and Specific Microplate Assay for the Determination of Intra- and Extracellular Ascorbate in Cultured Cells

Published on: April 11, 2014

13.9K

Related Experiment Videos

Last Updated: Jan 20, 2026

Preparation of Oligomeric β-amyloid1-42 and Induction of Synaptic Plasticity Impairment on Hippocampal Slices
04:41

Preparation of Oligomeric β-amyloid1-42 and Induction of Synaptic Plasticity Impairment on Hippocampal Slices

Published on: July 14, 2010

23.9K
Recording Synaptic Plasticity in Acute Hippocampal Slices Maintained in a Small-volume Recycling-, Perfusion-, and Submersion-type Chamber System
09:51

Recording Synaptic Plasticity in Acute Hippocampal Slices Maintained in a Small-volume Recycling-, Perfusion-, and Submersion-type Chamber System

Published on: January 1, 2018

12.1K
A Rapid and Specific Microplate Assay for the Determination of Intra- and Extracellular Ascorbate in Cultured Cells
11:56

A Rapid and Specific Microplate Assay for the Determination of Intra- and Extracellular Ascorbate in Cultured Cells

Published on: April 11, 2014

13.9K

Area of Science:

  • Neuroscience
  • Pharmacology
  • Biochemistry

Background:

  • Ascorbic acid (vitamin C) shows potential for rapid anxiolytic and antidepressant effects.
  • Ketamine is a fast-acting antidepressant, while fluoxetine is a conventional antidepressant.
  • Understanding the cellular mechanisms of rapid-acting antidepressants is crucial.

Purpose of the Study:

  • To investigate the effects of ascorbic acid, ketamine, and fluoxetine on anxiety- and depression-like behaviors.
  • To examine the impact of these treatments on hippocampal synaptic proteins, dendritic spine density, and morphology.
  • To compare the cellular mechanisms underlying the rapid effects of ascorbic acid and ketamine.

Main Methods:

  • Mice were administered ascorbic acid, ketamine, or fluoxetine.
  • Behavioral performance was assessed using the novelty suppressed feeding (NSF) test.
  • Hippocampal tissue was analyzed for synaptic protein immunocontent, dendritic spine density, morphology, and arborization.

Main Results:

  • Ascorbic acid and ketamine, unlike fluoxetine, reduced feeding latency in the NSF test.
  • Both ascorbic acid and ketamine increased p70S6K phosphorylation and, in ascorbic acid's case, synapsin I immunocontent.
  • Ascorbic acid and ketamine elevated dendritic spine density in the ventral dentate gyrus (DG), with ketamine also increasing it in the dorsal DG.

Conclusions:

  • Ascorbic acid's antidepressant and anxiolytic effects may stem from increased synaptic proteins and dendritic spine density in the ventral DG.
  • Ascorbic acid shares cellular mechanisms with ketamine, potentially enhancing synaptic function.
  • These findings support ascorbic acid as a potential rapid-acting therapeutic agent for mood disorders.