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Related Experiment Video

Updated: Jan 20, 2026

Isolation and Differentiation of Primary White and Brown Preadipocytes from Newborn Mice
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Dynamics of transcriptome changes during subcutaneous preadipocyte differentiation in ducks.

Zheng Wang1, Zhong-Tao Yin1, Fan Zhang1

  • 1National Engineering Laboratory for Animal Breeding and Key Laboratory of Animal Genetics, Breeding and Reproduction, MARA; Department of Animal Genetics and Breeding, China Agricultural University, Beijing, 100193, China.

BMC Genomics
|September 4, 2019
PubMed
Summary

This study reveals duck subcutaneous preadipocyte differentiation dynamics, identifying key transcription factors and pathways. Findings provide insights into fat deposition in Pekin ducks, crucial for animal agriculture.

Keywords:
Pekin duckPreadipocyte differentiationRegulation network modelSubcutaneous fatTranscription factorsTranscriptome

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Area of Science:

  • Animal Science
  • Molecular Biology
  • Genomics

Background:

  • Pekin ducks are a key animal model for studying fat synthesis and deposition.
  • Transcriptional regulation of adipose differentiation in ducks is not well understood.

Purpose of the Study:

  • To explore adipogenic transcriptional dynamics during proliferation and differentiation stages in duck subcutaneous preadipocytes.
  • To identify key regulatory factors and pathways involved in duck adipogenesis.

Main Methods:

  • Induction of duck subcutaneous preadipocyte differentiation using oleic acid.
  • Transcriptome analysis using 36 mRNA-seq libraries across 6 time points.
  • Differential gene expression analysis and weighted gene co-expression network analysis (WGCNA).

Main Results:

  • Identified thousands of differentially expressed genes during proliferation and differentiation.
  • Proliferation pathways were inhibited, while differentiation-initiating factors were activated.
  • WNT and FOXO pathways were negatively regulated, while adipogenic pathways were activated.
  • Over 100 transcription factors showed significant changes, including novel factors.
  • Proposed a regulatory network model, suggesting E2F1 as a key link between proliferation and differentiation.

Conclusions:

  • First comprehensive analysis of duck subcutaneous preadipocyte proliferation and differentiation transcriptomes.
  • Provides a foundation for understanding subcutaneous fat synthesis and deposition in ducks.
  • Identifies potential targets for future research on duck adipogenesis.