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Related Concept Videos

Using Sniper-Cas9 to Minimize Off-target Effects of CRISPR-Cas9 Without the Loss of On-target Activity Via Directed Evolution11:37

Using Sniper-Cas9 to Minimize Off-target Effects of CRISPR-Cas9 Without the Loss of On-target Activity Via Directed Evolution

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Here, we present a protocol to optimize CRISPR-Cas9 to achieve a higher specificity without the loss of on-target activity. We use a directed evolution approach called Sniper-screen to find a mutant Cas9 with the desired characteristics. Sniper-Cas9 is compatible with truncated single-guide RNAs and delivery in a ribonucleoprotein format, well-known strategies for achieving higher...
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Mutagenesis and Functional Selection Protocols for Directed Evolution of Proteins in E. coli09:01

Mutagenesis and Functional Selection Protocols for Directed Evolution of Proteins in E. coli

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Here we demonstrate a simple protocol to create a random mutant library for a given target sequence. We show how this method, which is performed in vivo in Escherichia coli, can be coupled with functional selections to evolve new enzymatic activities.
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All-in-One CRISPR Genome Editing: A Method for Homology Directed Repair-Based Gene Knock-In in Cultured Cells Using CRISPR-Cas9 System05:12

All-in-One CRISPR Genome Editing: A Method for Homology Directed Repair-Based Gene Knock-In in Cultured Cells Using CRISPR-Cas9 System

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In this video, we demonstrate all-in-one CRISPR-Cas9 based genome editing in cultured cells where Cas9 and sgRNA are provided as a single plasmid construct to the cells. The CRISPR-Cas9 system and desired gene to be inserted was introduced in cells through electroporation technique to facilitate successful gene...
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Directed Evolution Method in Saccharomyces cerevisiae: Mutant Library Creation and Screening10:50

Directed Evolution Method in Saccharomyces cerevisiae: Mutant Library Creation and Screening

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We present a detailed protocol to construct and screen mutant libraries for directed evolution campaigns in Saccharomyces cerevisiae.
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The Evidence for Evolution02:55

The Evidence for Evolution

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Genetic variations accumulating within populations over generations give rise to biological evolution. Evolutionary changes can result in the formation of novel varieties and entire new species. These changes are responsible for the diverse forms of life inhabiting the planet. The evidence for evolution suggests that all living organisms descended from common ancestors.
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Pooled CRISPR-Based Genetic Screens in Mammalian Cells09:05

Pooled CRISPR-Based Genetic Screens in Mammalian Cells

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CRISPR-Cas9 technology provides an efficient method to precisely edit the mammalian genome in any cell type and represents a novel means to perform genome-wide genetic screens. A detailed protocol discussing the steps required for the successful performance of pooled genome-wide CRISPR-Cas9 screens is provided...
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Related Experiment Video

Updated: Jan 20, 2026

Using Sniper-Cas9 to Minimize Off-target Effects of CRISPR-Cas9 Without the Loss of On-target Activity Via Directed Evolution
11:37

Using Sniper-Cas9 to Minimize Off-target Effects of CRISPR-Cas9 Without the Loss of On-target Activity Via Directed Evolution

Published on: February 26, 2019

10.3K

CRISPR-Based Directed Evolution for Crop Improvement.

Haroon Butt1, Syed Shan-E-Ali Zaidi2, Norhan Hassan1

  • 1Laboratory for Genome Engineering and Synthetic Biology, King Abdullah University of Science and Technology, Thuwal, 23955-6900, Saudi Arabia.

Trends in Biotechnology
|September 4, 2019
PubMed
Summary
This summary is machine-generated.

Directed evolution uses gene variants for desired traits. CRISPR-Cas9 enables targeted evolution for crop improvement, presenting new opportunities and challenges.

Keywords:
CRISPR-Cas9CRISPR-directed evolutionHDRNHEJcrop breedingtrait engineering

More Related Videos

Mutagenesis and Functional Selection Protocols for Directed Evolution of Proteins in E. coli
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Directed Evolution Method in Saccharomyces cerevisiae: Mutant Library Creation and Screening
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Directed Evolution Method in Saccharomyces cerevisiae: Mutant Library Creation and Screening

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Related Experiment Videos

Last Updated: Jan 20, 2026

Using Sniper-Cas9 to Minimize Off-target Effects of CRISPR-Cas9 Without the Loss of On-target Activity Via Directed Evolution
11:37

Using Sniper-Cas9 to Minimize Off-target Effects of CRISPR-Cas9 Without the Loss of On-target Activity Via Directed Evolution

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Mutagenesis and Functional Selection Protocols for Directed Evolution of Proteins in E. coli
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Mutagenesis and Functional Selection Protocols for Directed Evolution of Proteins in E. coli

Published on: March 16, 2011

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Directed Evolution Method in Saccharomyces cerevisiae: Mutant Library Creation and Screening
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Directed Evolution Method in Saccharomyces cerevisiae: Mutant Library Creation and Screening

Published on: April 1, 2016

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Area of Science:

  • Agricultural Science
  • Molecular Biology
  • Genetics

Background:

  • Directed evolution is a method for optimizing genes to achieve specific traits under selective pressures.
  • CRISPR-Cas9 gene editing technology offers precise genomic targeting capabilities.

Purpose of the Study:

  • To explore the potential of CRISPR-Cas9 in facilitating targeted directed evolution for crop enhancement.
  • To identify the opportunities and challenges associated with this novel approach in agriculture.

Main Methods:

  • Utilizing CRISPR-Cas9 systems for precise gene targeting.
  • Implementing selective pressures to drive the evolution of desired traits in crops.

Main Results:

  • CRISPR-Cas9 enables targeted generation of diverse gene variants for directed evolution.
  • This approach holds significant promise for accelerating crop improvement.

Conclusions:

  • CRISPR-Cas9-mediated targeted directed evolution is a powerful emerging platform for crop improvement.
  • Addressing associated challenges is crucial for realizing the full potential of this technology in agriculture.